Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1-benzyl-3-(hydroxymethyl-2-furyl)indazole | trivial name YC-1, allosteric activator, synergistically increases the catalytic activity in the presence of NO | Rattus norvegicus | |
N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) | NO donor | Rattus norvegicus | |
NO | binds at beta1 subunit heme, several hundred fold increase in activity | Rattus norvegicus | |
sodium nitroprusside | NO donor | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of sGC in Sf9 insect cells and COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C238S | no change in cGMP production | Rattus norvegicus |
C243S | no change in cGMP production | Rattus norvegicus |
C594D | no 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation, no synergy with NO | Rattus norvegicus |
C594D/E525K | loss of activity | Rattus norvegicus |
C594Y | reduced level of NO and 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation | Rattus norvegicus |
D477A | reduced level of NO and 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation | Rattus norvegicus |
M537N | high level of 1-benzyl-3-(hydroxymethyl-2-furyl)indazole activation | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
GTP | 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1 | Rattus norvegicus | |
0.039 | - |
GTP | 30°C, pH 8.0, in the presence of YC-1 | Rattus norvegicus | |
0.061 | - |
GTP | 30°C, pH 8.0, in the presence of N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-peniciiaminamide) | Rattus norvegicus | |
0.12 | - |
GTP | 30°C, pH 8.0, basal activity | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Rattus norvegicus | - |
3',5'-cyclic GMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant sGC | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
basal activity | Rattus norvegicus |
9.1 | - |
in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) | Rattus norvegicus |
12.1 | - |
in the presence of 0.1 mM N-(beta-D-glucopyranosyl-N2-acetyl-S-nitroso-D,L-penicillaminamide) and YC-1 | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Rattus norvegicus | 3',5'-cyclic GMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sGC | - |
Rattus norvegicus |
soluble guanylyl cyclase | - |
Rattus norvegicus |