Cloned (Comment) | Organism |
---|---|
into the pGEM-T vector for transformation of Escherichia coli JM109 cells, into pPIC9K for expression in Pichia pastoris | Nicotiana glutinosa |
Crystallization (Comment) | Organism |
---|---|
the crystal structures of RNase NT in complex with either 5'-AMP, 5'-GMP, or 2'-UMP are determined at 1.8 A resolutions by molecular replacement | Nicotiana glutinosa |
Protein Variants | Comment | Organism |
---|---|---|
C196T/C603T | double mutant to avoid restriction enzymatic scission | Nicotiana glutinosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana glutinosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nicotiana glutinosa |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease NT | - |
Nicotiana glutinosa |
RNase NT | - |
Nicotiana glutinosa |