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Literature summary for 4.6.1.24 extracted from

  • Kawano, S.; Kakuta, Y.; Nakashima, T.; Kimura, M.
    Crystal structures of the Nicotiana glutinosa ribonuclease NT in complex with nucleoside monophosphates (2006), J. Biochem., 140, 375-381.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into the pGEM-T vector for transformation of Escherichia coli JM109 cells, into pPIC9K for expression in Pichia pastoris Nicotiana glutinosa

Crystallization (Commentary)

Crystallization (Comment) Organism
the crystal structures of RNase NT in complex with either 5'-AMP, 5'-GMP, or 2'-UMP are determined at 1.8 A resolutions by molecular replacement Nicotiana glutinosa

Protein Variants

Protein Variants Comment Organism
C196T/C603T double mutant to avoid restriction enzymatic scission Nicotiana glutinosa

Organism

Organism UniProt Comment Textmining
Nicotiana glutinosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Nicotiana glutinosa

Synonyms

Synonyms Comment Organism
ribonuclease NT
-
Nicotiana glutinosa
RNase NT
-
Nicotiana glutinosa