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Literature summary for 4.8.1.4 extracted from
- High yield synthesis of nitriles by a new enzyme, phenylacetaldoxime dehydratase, from Bacillus sp. strain OxB-1 (2001), Biosci. Biotechnol. Biochem., 65, 2666-2672.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | high yield synthesis of 3-phenylpropionitrile from unpurified (E/Z)-3-phenylpropionaldoxime, which is spontaneously formed from 3-phenlypropionaldehyde and hydroxylamine in a butyl acetate/water biphasic system, production of further nitriles from their corresponding aldoximes | Bacillus sp. (in: Bacteria) |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. (in: Bacteria) | - |
enzyme expressed in Escherichia coli | - |
| Bacillus sp. (in: Bacteria) OxB-1 | - |
enzyme expressed in Escherichia coli | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Z)-3-phenylpropionaldoxime | - |
Bacillus sp. (in: Bacteria) | 3-phenylpropionitrile + H2O | quantitative yield of product | ? |  |
| (Z)-3-phenylpropionaldoxime | - |
Bacillus sp. (in: Bacteria) OxB-1 | 3-phenylpropionitrile + H2O | quantitative yield of product | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.3 | - |
Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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