Literature summary for 4.8.1.4 extracted from

Kobayashi, K.; Kubo, M.; Yoshioka, S.; Kitagawa, T.; Kato, Y.; Asano, Y.; Aono, S.
Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands (2006), Chembiochem, 7, 2004-2009.

Search for more literature for 4.8.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
amino-terminal His6-tagged H282G OxdB expressed in Escherichia coli	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
H282G	by site-directed mutagenesis	Bacillus sp. (in: Bacteria)
additional information	the H282G mutant has no enzymatic activity, the enzymatic activity is rescued by imidazole or pyridine derivatives that act as exogenous proximal ligand	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.38	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of pyridine	Bacillus sp. (in: Bacteria)
0.46	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 4-chloropyridine	Bacillus sp. (in: Bacteria)
0.47	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 4-methylimidazole	Bacillus sp. (in: Bacteria)
0.48	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 4-acetylpyridine	Bacillus sp. (in: Bacteria)
0.67	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 4-methylpyridine	Bacillus sp. (in: Bacteria)
0.7	-	(Z)-phenylacetaldoxime	wild type	Bacillus sp. (in: Bacteria)
0.76	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of imidazole	Bacillus sp. (in: Bacteria)
0.78	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 1-methylimidazole	Bacillus sp. (in: Bacteria)
1.1	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 2-methylimidazole	Bacillus sp. (in: Bacteria)
1.64	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 4-methoxypyridine	Bacillus sp. (in: Bacteria)
2.52	-	(Z)-phenylacetaldoxime	H282G mutant in the presence of 1-vinylimidazole	Bacillus sp. (in: Bacteria)

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	P82604	-	-
Bacillus sp. (in: Bacteria) OxB-1	P82604	-	-

Purification (Commentary)

Purification (Comment)	Organism
by Co2+-charged metal-ion-chelating Talon column chromatography	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(Z)-phenylacetaldehyde oxime	-	Bacillus sp. (in: Bacteria)	phenylacetonitrile + H2O	-	?
(Z)-phenylacetaldehyde oxime	-	Bacillus sp. (in: Bacteria) OxB-1	phenylacetonitrile + H2O	-	?
(Z)-phenylacetaldoxime	-	Bacillus sp. (in: Bacteria)	phenylacetonitrile + H2O	-	?
(Z)-phenylacetaldoxime	-	Bacillus sp. (in: Bacteria) OxB-1	phenylacetonitrile + H2O	-	?

Synonyms

Synonyms	Comment	Organism
phenylacetaldoxime dehydratase	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)