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Literature summary for 4.8.1.4 extracted from

  • Raedisch, R.; Chmatal, M.; Rucka, L.; Novotny, P.; Petraskova, L.; Halada, P.; Kotik, M.; Patek, M.; Martinkova, L.
    Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in Bradyrhizobium sp. (2018), Int. J. Biol. Macromol., 115, 746-753 .
    View publication on PubMed
Search for more literature for 4.8.1.4

Activating Compound

Activating Compound Comment Organism Structure
Na2S2O4 the addition of Na2S2O4 (5 mM) is essential for the activity of the enzyme Bradyrhizobium sp. LTSPM299

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bradyrhizobium sp. LTSPM299
expression in Escherichia coli Bradyrhizobium sp. LTSPM299

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40700
-
 gel filtration Bradyrhizobium sp. LTSPM299

Organism

Organism UniProt Comment Textmining
Bradyrhizobium sp. LTSPM299 A0A0D7Q864
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Bradyrhizobium sp. LTSPM299

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E/Z)-2-phenylpropionaldoxime 17% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 2-phenylpropiononitrile + H2O
-
 ?
(E/Z)-2-phenylpropionaldoxime
-
 Bradyrhizobium sp. LTSPM299 (E/Z)-2-phenylpropiononitrile + H2O
-
 ?
(E/Z)-3-phenylpropionaldoxime
-
 Bradyrhizobium sp. LTSPM299 3-phenylpropiononitrile + H2O
-
 ?
(E/Z)-3-phenylpropionaldoxime 131% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 3-phenylpropiononitrile + H2O
-
 ?
(E/Z)-butyraldoxime
-
 Bradyrhizobium sp. LTSPM299 butyronitrile + H2O
-
 ?
(E/Z)-indole-3-acetaldoxime
-
 Bradyrhizobium sp. LTSPM299 indole-3-acetonitrile + H2O
-
 ?
(E/Z)-indole-3-acetaldoxime 10% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 indole-3-acetonitrile + H2O
-
 ?
(E/Z)-isovaleraldoxime
-
 Bradyrhizobium sp. LTSPM299 isovaleronitrile + H2O
-
 ?
(E/Z)-isovaleraldoxime 14% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 isovaleronitrile + H2O
-
 ?
(E/Z)-n-butyraldoxime 12% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 n-butyronitrile + H2O
-
 ?
(E/Z)-n-valeraldoxime 55% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 n-valeronitrile + H2O
-
 ?
(E/Z)-phenylacetaldehyde oxime
-
 Bradyrhizobium sp. LTSPM299 phenylacetonitrile + H2O
-
 ?
(E/Z)-phenylacetaldoxime
-
 Bradyrhizobium sp. LTSPM299 phenylacetonitrile + H2O
-
 ?
(E/Z)-propionaldoxime
-
 Bradyrhizobium sp. LTSPM299 propionitrile + H2O
-
 ?
(E/Z)-propionaldoxime 5% of the activity with (E/Z)-phenylacetaldehyde oxime Bradyrhizobium sp. LTSPM299 propionitrile + H2O
-
 ?
(E/Z)-valeraldoxime
-
 Bradyrhizobium sp. LTSPM299 valeronitrile + H2O
-
 ?
(Z)-phenylacetaldehyde oxime
-
 Bradyrhizobium sp. LTSPM299 phenylacetonitrile + H2O
-
 ?
additional information the enzyme is inactive with (hetero)aromatic aldoximes ((E)-pyridine-2-aldoxime, (E/Z)-pyridine-3-aldoxime, (E/Z)-pyridine-4-aldoxime, and (E/Z)-benzaldoxime) Bradyrhizobium sp. LTSPM299 ?
-
-

Subunits

Subunits Comment Organism
monomer 1 * 40700, calculated from sequence, 1 * 40000, SDS-PAGE, His-tagged recombinant protein Bradyrhizobium sp. LTSPM299
monomer 1 * 40000, His6-tagged enzyme, SDS-PAGE Bradyrhizobium sp. LTSPM299
monomer 1 * 40700, calculated from amino acid sequence Bradyrhizobium sp. LTSPM299

Synonyms

Synonyms Comment Organism
OxdBr1
-
 Bradyrhizobium sp. LTSPM299
UP10_21065
-
 Bradyrhizobium sp. LTSPM299

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bradyrhizobium sp. LTSPM299

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable up to Bradyrhizobium sp. LTSPM299
30
-
 the enzyme is unstable when exposed to temperatures exceeding 30°C Bradyrhizobium sp. LTSPM299

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Bradyrhizobium sp. LTSPM299

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5
-
 Bradyrhizobium sp. LTSPM299
5 7.5 the enzyme is active over a pH range from ca. 5.0 to 7.5 Bradyrhizobium sp. LTSPM299

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8
-
 Bradyrhizobium sp. LTSPM299
7 8 the enzyme stability is the highest at pH ca. 7.0-8.0 Bradyrhizobium sp. LTSPM299