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Literature summary for 4.8.1.4 extracted from

  • Kato, Y.; Asano, Y.
    Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum (2005), Biosci. Biotechnol. Biochem., 69, 2254-2257 .
    View publication on PubMed
Search for more literature for 4.8.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Fusarium graminearum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.802
-
 (E/Z)-n-capronaldoxime at pH 7.0 and 25°C Fusarium graminearum
1.46
-
 (E/Z)-indoleacetaldoxime at pH 7.0 and 25°C Fusarium graminearum
1.7
-
 (E/Z)-mandelaldoxime at pH 7.0 and 25°C Fusarium graminearum
1.79
-
 (E/Z)-4-phenylbutyraldoxime at pH 7.0 and 25°C Fusarium graminearum
2.66
-
 (E/Z)-isovaleraldoxime at pH 7.0 and 25°C Fusarium graminearum
2.76
-
 (Z)-3-phenylpropionaldoxime at pH 7.0 and 25°C Fusarium graminearum
2.87
-
 (E/Z)-n-butyraldoxime at pH 7.0 and 25°C Fusarium graminearum
3.52
-
 (Z)-phenylacetaldoxime at pH 7.0 and 25°C Fusarium graminearum
3.71
-
 (E/Z)-2-phenylpropionaldoxime at pH 7.0 and 25°C Fusarium graminearum
10.1
-
 (E/Z)-n-valeraldoxime at pH 7.0 and 25°C Fusarium graminearum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34100
-
 native enzyme, gel filtration Fusarium graminearum

Organism

Organism UniProt Comment Textmining
Fusarium graminearum Q2WG72
-
-
Fusarium graminearum MAFF305135 Q2WG72
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.8
-
 at pH 7.0 and 25°C Fusarium graminearum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E/Z)-2-phenylpropionaldoxime 68.6% activity compared to Z-phenylacetaldoxime Fusarium graminearum (E/Z)-2-phenylpropiononitrile + H2O
-
 ?
(E/Z)-4-phenylbutyraldoxime 79.2% activity compared to Z-phenylacetaldoxime Fusarium graminearum phenylbutyronitrile + H2O
-
 ?
(E/Z)-cyclohexanecarboxaldehyde oxime 11.6% activity compared to Z-phenylacetaldoxime Fusarium graminearum ? + H2O
-
 ?
(E/Z)-indoleacetaldoxime 125% activity compared to Z-phenylacetaldoxime Fusarium graminearum (E/Z)-indoleacetonitrile + H2O
-
 ?
(E/Z)-isobutyraldoxime 5.97% activity compared to Z-phenylacetaldoxime Fusarium graminearum ? + H2O
-
 ?
(E/Z)-isovaleraldoxime 92.7% activity compared to Z-phenylacetaldoxime Fusarium graminearum isovaleronitrile + H2O
-
 ?
(E/Z)-isovaleraldoxime 92.7% activity compared to Z-phenylacetaldoxime Fusarium graminearum MAFF305135 isovaleronitrile + H2O
-
 ?
(E/Z)-mandelaldoxime 16.1% activity compared to Z-phenylacetaldoxime Fusarium graminearum (E/Z)-mandelacetonitrile + H2O
-
 ?
(E/Z)-n-butyraldoxime 107% activity compared to Z-phenylacetaldoxime Fusarium graminearum n-butyronitrile + H2O
-
 ?
(E/Z)-n-butyraldoxime 107% activity compared to Z-phenylacetaldoxime Fusarium graminearum MAFF305135 n-butyronitrile + H2O
-
 ?
(E/Z)-n-capronaldoxime 17.6% activity compared to Z-phenylacetaldoxime Fusarium graminearum n-caprononitrile + H2O
-
 ?
(E/Z)-n-capronaldoxime 17.6% activity compared to Z-phenylacetaldoxime Fusarium graminearum MAFF305135 n-caprononitrile + H2O
-
 ?
(E/Z)-n-valeraldoxime 341% activity compared to Z-phenylacetaldoxime Fusarium graminearum n-valeronitrile + H2O
-
 ?
(E/Z)-n-valeraldoxime 341% activity compared to Z-phenylacetaldoxime Fusarium graminearum MAFF305135 n-valeronitrile + H2O
-
 ?
(E/Z)-propionaldoxime 10.6% activity compared to Z-phenylacetaldoxime Fusarium graminearum ? + H2O
-
 ?
(Z)-3-phenylpropionaldoxime 104% activity compared to Z-phenylacetaldoxime Fusarium graminearum (Z)-3-phenylpropiononitrile + H2O
-
 ?
(Z)-cinnamaldehyde oxime 17.9% activity compared to Z-phenylacetaldoxime Fusarium graminearum ? + H2O
-
 ?
(Z)-phenylacetaldoxime 100% activity Fusarium graminearum phenylacetonitrile + H2O
-
 ?
(Z)-phenylacetaldoxime 100% activity Fusarium graminearum MAFF305135 phenylacetonitrile + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 44070, calculated from amino acid sequence Fusarium graminearum

Synonyms

Synonyms Comment Organism
OxdFG
-
 Fusarium graminearum
phenylacetaldoxime dehydratase
-
 Fusarium graminearum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Fusarium graminearum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 the enzyme is stable below 20°C Fusarium graminearum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Fusarium graminearum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8 the enzyme is stable at pH 4.5-8.0 Fusarium graminearum