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Literature summary for 4.8.1.4 extracted from

  • Chen, Z.; Mao, F.; Zheng, H.; Xiao, Q.; Ding, Z.; Wang, A.; Pei, X.
    Cyanide-free synthesis of aromatic nitriles from aldoximes Discovery and application of a novel heme-containing aldoxime dehydratase (2021), Enzyme Microb. Technol., 150, 109883 .
    View publication on PubMed
Search for more literature for 4.8.1.4

Activating Compound

Activating Compound Comment Organism Structure
Na2S2O4 the enzyme activity is significantly improved with the addition of Na2S2O4 as a strong deducing reagent Pseudomonas putida

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

General Stability

General Stability Organism
the enzyme completely loses the catalytic activity in 0.1 M glycine-sodium hydroxide buffer (pH 8.4) Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.25
-
 3-pyridine aldoxime at pH 6.0 and 35°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1E)-N-hydroxyethanimine more than 99.9% conversion Pseudomonas putida acetonitrile + H2O
-
 ?
(1E)-N-hydroxyheptan-1-imine more than 99.9% conversion Pseudomonas putida heptanenitrile + H2O
-
 ?
(1E)-N-hydroxyhexan-1-imine more than 99.9% conversion Pseudomonas putida hexanenitrile + H2O
-
 ?
(E)-1-(2-chloro-6-fluorophenyl)-N-hydroxymethanimine 42.7% conversion Pseudomonas putida 2-chloro-6-fluorobenzonitrile + H2O
-
 ?
(E)-N-hydroxy-1-(pyridin-4-yl)methanimine 12.3% conversion Pseudomonas putida pyridine-4-carbonitrile + H2O
-
 ?
(E)-N-hydroxy-1-(thiophen-2-yl)methanimine 99.5% conversion Pseudomonas putida thiophene-2-carbonitrile + H2O
-
 ?
(E)-N-hydroxy-1-phenylmethanimine more than 99.9% conversion Pseudomonas putida benzonitrile + H2O
-
 ?
(Z)-1-(2-bromophenyl)-N-hydroxymethanimine more than 99.9% conversion Pseudomonas putida 2-bromobenzonitrile + H2O
-
 ?
(Z)-N-hydroxy-1-(pyridin-2-yl)methanimine 99.8% conversion Pseudomonas putida pyridine-2-carbonitrile + H2O
-
 ?
2-[(E)-(hydroxyimino)methyl]phenol 19% conversion Pseudomonas putida 2-hydroxybenzonitrile + H2O
-
 ?
3-pyridine aldoxime more than 99.9% conversion Pseudomonas putida pyridine-3-carbonitrile + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas putida
? x * 41600, calculated from amino acid sequence Pseudomonas putida

Synonyms

Synonyms Comment Organism
OxdF1
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 40 the enzymatic activity remains above 80% of the maximal activity between 30 and 40°C Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 35 the residual activity of recombinant enzyme maintains above 70% of the initial activity after 3 h at 30°C. When the enzyme is incubated at 35°C, about 20% of the initial activity is retained after 1 h. The half-lives of recombinant enzyme are calculated as 3.8 h and 46 min at 30 and 35°C h, respectively Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.873
-
 3-pyridine aldoxime at pH 6.0 and 35°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FMN necessarily required Pseudomonas putida
heme the enzyme carries protoheme IX as the prosthetic group Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2683
-
 3-pyridine aldoxime at pH 6.0 and 35°C Pseudomonas putida