Cloned (Comment) | Organism |
---|---|
overexpressed as a C-terminal His6-tag protein in Escherichia coli BL21 (DE3) | Brevibacillus brevis |
Protein Variants | Comment | Organism |
---|---|---|
C331A | minor changes in activity or specificity | Brevibacillus brevis |
C331L | activity of GrsA is decreased to 26% | Brevibacillus brevis |
C376S | no changes in activity | Brevibacillus brevis |
C473A | no changes in activity | Brevibacillus brevis |
C60F | no changes in activity | Brevibacillus brevis |
C60F/C331A/C376S/C473A | named A-PCP(delta-4Cys), Km for L-Phe is increased by 2fold, while the kcat is reduced by 1.5fold | Brevibacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
L-Phe | - |
Brevibacillus brevis | |
0.0166 | - |
L-Phe | mutant A-PCP(delta-4Cys) | Brevibacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73900 | - |
holo-A-PCP-form (GrsA plus peptidyl carrier protein domain), gel-filtration | Brevibacillus brevis |
74200 | - |
apo-A-PCP-form (GrsA plus peptidyl carrier protein domain), gel-filtration | Brevibacillus brevis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevibacillus brevis | P0C062 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-Phe + H2O | - |
Brevibacillus brevis | AMP + diphosphate + D-Phe | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Gramicidin S synthetase I | - |
Brevibacillus brevis |
GrsA | - |
Brevibacillus brevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
L-Phe | mutant A-PCP(delta-4Cys) | Brevibacillus brevis | |
0.38 | - |
L-Phe | - |
Brevibacillus brevis |