Protein Variants | Comment | Organism |
---|---|---|
additional information | wild-type enzyme consists of two identical subunits that form a very tight dimer involving interactions of 32 residues of each subunit. By replacing 15 residues of the major interface loop by another 8-residue fragment, a variant is constructed that is a stable and monomeric protein with TIM activity, monoTIM. The turnover numer of the monomeric form is 100fold lower | Trypanosoma brucei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | 25360 | genetically engineered monomeric enzyme form, gel filtration, sedimentation equilibrium experiments | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type enzyme consists of two identical subunits that form a very tight dimer involving interactions of 32 residues of each subunit | Trypanosoma brucei |