Protein Variants | Comment | Organism |
---|---|---|
E186D | mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum | Actinoplanes missouriensis |
E186Q | mutant enzymes E186D and E186Q are active, and their metal specificity is different from that of the wild type. The E186 enzyme is most active with Mn2+ and has a drastically shifted pH optimum | Actinoplanes missouriensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for wild-type enzyme and mutants E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ | Actinoplanes missouriensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | Mg2+, Mn2+ or Co2+ required for maximal activity | Actinoplanes missouriensis | |
Mg2+ | - |
Actinoplanes missouriensis | |
Mn2+ | - |
Actinoplanes missouriensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes missouriensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | - |
Actinoplanes missouriensis | D-Xylulose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers for wild-type enzyme and mutant enzymes E186D and E186Q, activated by Mg2+, Mn2+ or Co2+ | Actinoplanes missouriensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.25 | - |
mutant E186Q, xylose isomerization, activated by Mn2+ or Mg2+ | Actinoplanes missouriensis |
7.3 | - |
wild-type enzyme, xylose isomerization, activated by Mg2+ | Actinoplanes missouriensis |
7.9 | - |
wild-type enzyme, xylose isomerization, activated by Mg2+ | Actinoplanes missouriensis |