Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli HB101 | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E372G | broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type | Thermus thermophilus |
E372G/F163L | broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type | Thermus thermophilus |
E372G/V379A | broader pH range and nine times higher turnover for D-xylose at 60°C than wild-type | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
xylitol | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.44 | - |
D-xylose | 60°C, pH 7, wild-type | Thermus thermophilus | |
25.1 | - |
D-xylose | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus | |
28.7 | - |
D-xylose | 60°C, pH 7, mutant E372G | Thermus thermophilus | |
52 | - |
D-glucose | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus | |
89.4 | - |
D-xylose | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus | |
130.8 | - |
D-glucose | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus | |
146.8 | - |
D-glucose | 60°C, pH 7, wild-type | Thermus thermophilus | |
171.8 | - |
D-glucose | 60°C, pH 7, mutant E372G | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Thermus thermophilus | |
Mg2+ | - |
Thermus thermophilus | |
Mn2+ | - |
Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glucose | - |
Thermus thermophilus | D-Fructose | - |
r | |
D-Xylose | - |
Thermus thermophilus | D-Xylulose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
XI | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
mutants E372G/V379A | Thermus thermophilus |
90 | - |
wild-type | Thermus thermophilus |
95 | - |
mutants E372G/F163L and E372G | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-glucose | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus | |
0.833 | - |
D-glucose | 60°C, pH 7, wild-type | Thermus thermophilus | |
16.3 | - |
D-glucose | 60°C, pH 7, wild-type | Thermus thermophilus | |
23.8 | - |
D-glucose | 60°C, pH 7, mutant E372G | Thermus thermophilus | |
39.9 | - |
D-glucose | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus | |
46.6 | - |
D-xylose | 60°C, pH 7, wild-type | Thermus thermophilus | |
66.9 | - |
D-glucose | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus | |
70.8 | - |
D-xylose | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus | |
88.7 | - |
D-glucose | 60°C, pH 7, mutant E372G | Thermus thermophilus | |
257.5 | - |
D-xylose | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus | |
258 | - |
D-xylose | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | mutants E372G/V379A, E372G/F163L and E372G | Thermus thermophilus |
5 | 8 | wild-type | Thermus thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
xylitol | 60°C, pH 7, wild-type | Thermus thermophilus | |
33.2 | - |
xylitol | 60°C, pH 7, mutant E372G/V379A | Thermus thermophilus | |
68.7 | - |
xylitol | 60°C, pH 7, mutant E372G | Thermus thermophilus | |
1174 | - |
xylitol | 60°C, pH 7, mutant E372G/F163L | Thermus thermophilus |