Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structure of the enzyme complexed with p-hydroxyphenylpyruvate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
P1(A)M2 | insertion abolishes activity | Homo sapiens |
P1G | mutation substantially reduces the catalytic activity | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12345 | - |
x * 12345, wild-type enzyme, mass spectrometry | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | important immunoregulatory molecule with a unique ability to suppress the anti-inflammatory effects of glucocorticoids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14174 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
keto-(p-hydroxyphenyl)pyruvate | the substrate interacts with Pro1, Lys32 and Ile64 from one subunit and Tyr95 and Asn97 from an adjacent subunit | Homo sapiens | enol-(p-hydroxyphenyl)pyruvate | - |
? | |
additional information | important immunoregulatory molecule with a unique ability to suppress the anti-inflammatory effects of glucocorticoids | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 12345, wild-type enzyme, mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MIF | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
keto-(p-hydroxyphenyl)pyruvate | 25°C, pH 6.0, mutant enzyme P1(A)M2 | Homo sapiens | |
0.93 | - |
keto-(p-hydroxyphenyl)pyruvate | 25°C, pH 6.0, mutant enzyme P1G | Homo sapiens | |
2.7 | - |
keto-(p-hydroxyphenyl)pyruvate | 25°C, pH 6.0, wild-type enzyme | Homo sapiens | |
6.08 | - |
keto-(p-hydroxyphenyl)pyruvate | 25°C, pH 6.0, mutant enzyme P1G | Homo sapiens |