Protein Variants | Comment | Organism |
---|---|---|
P29G | kcat-value is 1.6fold lower than wild-type value. KM-value is 2.9fold higher than wild-type value | Escherichia coli |
P29L | kcat-value is 1.9fold lower than wild-type value. KM-value is 4.2fold higher than wild-type value | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000108 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli | |
0.000318 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
0.000455 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AA37 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tRNA uridine32 | - |
Escherichia coli | tRNA pseudouridine32 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
Tm-value for mutant enzyme P29L | Escherichia coli |
38 | - |
Tm-value for mutant enzyme P29G is 37.9°C | Escherichia coli |
45 | - |
Tm-value for wild-type enzyme is 44.9°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli | |
0.061 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
0.099 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
112.1 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29L | Escherichia coli | |
191.8 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, mutant enzyme P29G | Escherichia coli | |
916.7 | - |
tRNA uridine32 | pH 7.5, 37°C, Escherichia coli tRNAPhe, wild-type enzyme | Escherichia coli |