Literature summary for 5.4.99.61 extracted from

Shipman, L.W.; Li, D.; Roessner, C.A.; Scott, A.I.; Sacchettini, J.C.
Crystal structure of precorrin-8x methyl mutase (2001), Structure, 9, 587-596.

Search for more literature for 5.4.99.61

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas denitrificans (nom. rej.)

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo form and complexed with hydrogenobirinate, hanging drop vapor diffusion with equal volumes of concentrated protein, i.e. 10 mg/ml, and a precipitant solution containing 25%-32.5% polyethylene glycol 4000, 200 mM MgCl2, 100 mM Tris-HCl, pH 8.5, crystals diffract to 2.1 A and 2.6 A resolution	Pseudomonas denitrificans (nom. rej.)

Inhibitors

Inhibitors	Comment	Organism	Structure
Hydrogenobyrinate	-	Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00091	-	Hydrogenobyrinate	-	Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22060	-	gel filtration, native-PAGE	Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Precorrin-8X	Pseudomonas denitrificans (nom. rej.)	involved in the aerobic pathway to vitamin B12	Hydrogenobyrinate	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas denitrificans (nom. rej.)	P21638	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant CoBH	Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Precorrin-8X	-	Pseudomonas denitrificans (nom. rej.)	Hydrogenobyrinate	-	?
Precorrin-8X	involved in the aerobic pathway to vitamin B12	Pseudomonas denitrificans (nom. rej.)	Hydrogenobyrinate	-	?

Synonyms

Synonyms	Comment	Organism
CoBH	-	Pseudomonas denitrificans (nom. rej.)

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00017	-	Hydrogenobyrinate	product inhibition	Pseudomonas denitrificans (nom. rej.)

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Release 2023.1 (January 2023)