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Literature summary for 5.5.1.1 extracted from

  • Camara, B.; Bielecki, P.; Kaminski, F.; dos Santos, V.M.; Plumeier, I.; Nikodem, P.; Pieper, D.H.
    A gene cluster involved in degradation of substituted salicylates via ortho cleavage in Pseudomonas sp. strain MT1 encodes enzymes specifically adapted for transformation of 4-methylcatechol and 3-methylmuconate (2007), J. Bacteriol., 189, 1664-1674.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
part of gene cluster involved in degradation of substituted salicylates Pseudomonas sp.
part of gene cluster involved in degradation of substituted salicylates Pseudomonas reinekei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
 3-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
0.026
-
 3-methyl-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
0.0306
-
 2-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
0.031
-
 cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
0.099
-
 3-chloro-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
0.41
-
 3-chloro-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
1.73
-
 cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40248
-
 x * 40248, calculated Pseudomonas reinekei
41076
-
 x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.
42000
-
 x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas reinekei Q4KPK2 isoform SalC
-
Pseudomonas reinekei MT1 Q4KPK2 isoform SalC
-
Pseudomonas sp.
-
 isoform CatB
-
Pseudomonas sp. MT1
-
 isoform CatB
-

Purification (Commentary)

Purification (Comment) Organism
purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells Pseudomonas sp.
purified from cell extracts of salicylate-, 4-methylsalicylate-, or 5-chlorosalicylate-grown cells Pseudomonas reinekei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-cis,cis-muconate
-
 Pseudomonas sp. 5-chloromuconolactone + 2-chloromuconolactone
-
 ?
2-chloro-cis,cis-muconate
-
 Pseudomonas reinekei 5-chloromuconolactone + 2-chloromuconolactone
-
 ?
2-chloro-cis,cis-muconate
-
 Pseudomonas reinekei MT1 5-chloromuconolactone + 2-chloromuconolactone
-
 ?
2-chloro-cis,cis-muconate
-
 Pseudomonas sp. MT1 5-chloromuconolactone + 2-chloromuconolactone
-
 ?
2-methyl-cis,cis-muconate
-
 Pseudomonas sp. 2-methylmuconolactone
-
 ?
2-methyl-cis,cis-muconate
-
 Pseudomonas reinekei 2-methylmuconolactone
-
 ?
2-methyl-cis,cis-muconate
-
 Pseudomonas reinekei MT1 2-methylmuconolactone
-
 ?
2-methyl-cis,cis-muconate
-
 Pseudomonas sp. MT1 2-methylmuconolactone
-
 ?
3-Chloro-cis,cis-muconate
-
 Pseudomonas sp. 5-Chloromuconolactone
-
 ?
3-Chloro-cis,cis-muconate
-
 Pseudomonas reinekei 5-Chloromuconolactone
-
 ?
3-Chloro-cis,cis-muconate
-
 Pseudomonas reinekei MT1 5-Chloromuconolactone
-
 ?
3-Chloro-cis,cis-muconate
-
 Pseudomonas sp. MT1 5-Chloromuconolactone
-
 ?
3-methyl-cis,cis-muconate
-
 Pseudomonas sp. 4-methylmuconolactone
-
 ?
3-methyl-cis,cis-muconate
-
 Pseudomonas reinekei 4-methylmuconolactone
-
 ?
3-methyl-cis,cis-muconate
-
 Pseudomonas reinekei MT1 4-methylmuconolactone
-
 ?
3-methyl-cis,cis-muconate
-
 Pseudomonas sp. MT1 4-methylmuconolactone
-
 ?
cis,cis-Muconate
-
 Pseudomonas sp. (+)-Muconolactone
-
 ?
cis,cis-Muconate
-
 Pseudomonas reinekei (+)-Muconolactone
-
 ?
cis,cis-Muconate
-
 Pseudomonas reinekei MT1 (+)-Muconolactone
-
 ?
cis,cis-Muconate
-
 Pseudomonas sp. MT1 (+)-Muconolactone
-
 ?

Subunits

Subunits Comment Organism
? x * 40248, calculated Pseudomonas reinekei
? x * 41076, calculated, x * 42000, SDS-PAGE Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 2-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
14.4
-
 3-chloro-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
27.8
-
 3-methyl-cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
120
-
 3-chloro-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
125
-
 cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei
186
-
 cis,cis-muconate isoform CatB, 25°C Pseudomonas sp.
186
-
 cis,cis-muconate isoform CatB, 25°C Pseudomonas reinekei
214
-
 3-methyl-cis,cis-muconate isoform SalC, 25°C Pseudomonas reinekei