Cloned (Comment) | Organism |
---|---|
arginyl tRNA synthetase and an N-terminally truncated derivative with a deletion of the 73 N-terminal amino acids, expression in Escherichia coli | Cricetulus sp. |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-73 | KM-value for tRNAArg is 1.2fold higher than the wild-type value, turnover number is 1.4fold higher | Cricetulus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00032 | - |
tRNAArg | 25°C, pH 7.5, native tRNAArg from beef liver, wild type enzyme | Cricetulus sp. | |
0.00039 | - |
tRNAArg | 25°C, pH 7.5, native tRNAArg from beef liver, mutant enzyme DELTA1-73 | Cricetulus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-arginine + tRNAArg | - |
Cricetulus sp. | AMP + diphosphate + L-arginyl-tRNAArg | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
tRNAArg | 25°C, pH 7.5, native tRNAArg from beef liver, wild type enzyme | Cricetulus sp. | |
2.6 | - |
tRNAArg | 25°C, pH 7.5, native tRNAArg from beef liver, mutant enzyme DELTA1-73 | Cricetulus sp. |