Cloned (Comment) | Organism |
---|---|
expressionof wild-type and mutant enzymes in Escherichia coli strain JM109 | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants with decreased activity by deletion of residues 108-122, causing loss of 44% activity, or 234-238, causing loss of 80% activity, or both parts, resulting in an inactive mutant enzyme, deletion of residues 234-238 affected the normally induced expression at 37°C | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00018 | - |
tRNATrp | pH 7.5, 22°C, wild-type enzyme | Bacillus subtilis | |
0.00022 | - |
tRNATrp | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
0.00052 | - |
tRNATrp | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis | |
0.00073 | - |
L-tryptophan | pH 7.5, 22°C, wild-type enzyme | Bacillus subtilis | |
0.0021 | - |
L-tryptophan | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
0.0023 | - |
L-tryptophan | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Bacillus subtilis | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and mutants from Escherichia coli JM109, to homogeneity | Bacillus subtilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-tryptophan + tRNATrp = AMP + diphosphate + L-tryptophyl-tRNATrp | 2-step reaction, tRNATrp substrate recognition, mechanism and modeling | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Bacillus subtilis | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | 2-step reaction, tRNATrp substrate recognition, mechanism and modeling | Bacillus subtilis | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
additional information | enzyme also performs the ATP-diphosphate exchange reaction | Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Bacillus subtilis |
Tryptophanyl-tRNA synthase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Bacillus subtilis |
25 | - |
exchange reaction assay at | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.59 | - |
L-tryptophan | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis | |
0.59 | - |
tRNATrp | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis | |
0.61 | - |
L-tryptophan | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
0.61 | - |
tRNATrp | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
0.9 | - |
L-tryptophan | pH 7.5, 22°C, wild-type enzyme | Bacillus subtilis | |
0.9 | - |
tRNATrp | pH 7.5, 22°C, wild-type enzyme | Bacillus subtilis | |
6.08 | - |
L-tryptophan | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
6.08 | - |
tRNATrp | pH 7.5, 22°C, deletion 108-122 mutant | Bacillus subtilis | |
6.08 | - |
L-tryptophan | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis | |
6.08 | - |
tRNATrp | pH 7.5, 22°C, deletion 234-238 mutant | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |