Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens | |
heme | R135H/E438A double mutant | Bos taurus | |
Zn2+ | - |
Homo sapiens | |
Zn2+ | R135H/E438A double mutant | Bos taurus |
Cloned (Comment) | Organism |
---|---|
into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Mus musculus |
into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Bos taurus |
into the vector pET20b for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E438A | TrpRS single mutant | Bos taurus |
H130R | mutant, constitutively active | Homo sapiens |
R135H | TrpRS single mutant | Bos taurus |
R135H/E438A | TrpRS double mutant, binds with Zn2+ or heme to enhance its aminoacylation activity | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Bos taurus | |
Zn2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Mus musculus | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | Bos taurus | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | P23381 | - |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on a nickel affinity column | Mus musculus |
on a nickel affinity column | Bos taurus |
on a nickel affinity column | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | - |
Mus musculus | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | - |
Bos taurus | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophyl-tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Mus musculus |
TrpRS | - |
Bos taurus |
TrpRS | - |
Homo sapiens |
Tryptophanyl-tRNA synthetase | - |
Mus musculus |
Tryptophanyl-tRNA synthetase | - |
Bos taurus |
Tryptophanyl-tRNA synthetase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
aminoacylation assay | Mus musculus |
20 | - |
aminoacylation assay | Bos taurus |
20 | - |
aminoacylation assay | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
aminoacylation assay | Mus musculus |
7.5 | - |
aminoacylation assay | Bos taurus |
7.5 | - |
aminoacylation assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus | |
ATP | - |
Bos taurus | |
ATP | - |
Homo sapiens |