Crystallization (Comment) | Organism |
---|---|
native enzyme complexed with phenylalanyl-adenylate in presence of manganese, X-ray diffraction structure determination at 2.6 A resolution and analysis | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | not bound to the active site, located at the interface of alpha and beta subunits | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | Thermus thermophilus | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
class II enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe | reaction mechanism | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-phenylalanine + tRNAPhe | - |
Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
ATP + L-phenylalanine + tRNAPhe | two-step reaction, the first step, formation of phenylalanyl-adenylate intermediate, proceeds also within crystals, where the intermediate is bound to the active site involving neighbouring residues Phe258 and Phe260 | Thermus thermophilus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
additional information | the enzyme probably interacts with DNA | Thermus thermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CML33 | - |
Thermus thermophilus |
FRS | - |
Thermus thermophilus |
HSPC173 | - |
Thermus thermophilus |
L-Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
Phenylalanine translase | - |
Thermus thermophilus |
Phenylalanine--tRNA ligase | - |
Thermus thermophilus |
Phenylalanine-tRNA synthetase | - |
Thermus thermophilus |
Phenylalanyl transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Phenylalanyl-transfer ribonucleate synthetase | - |
Thermus thermophilus |
Phenylalanyl-transfer RNA ligase | - |
Thermus thermophilus |
Phenylalanyl-transfer RNA synthetase | - |
Thermus thermophilus |
Phenylalanyl-tRNA ligase | - |
Thermus thermophilus |
Phenylalanyl-tRNA synthetase | - |
Thermus thermophilus |
PheRS | - |
Thermus thermophilus |
Synthetase, phenylalanyl-transfer ribonucleate | - |
Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermus thermophilus |