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Literature summary for 6.1.1.20 extracted from
- Crystallization and X-ray analysis of human cytoplasmic phenylalanyl-tRNA synthetase (2009), Acta Crystallogr. Sect. F, 65, 93-97.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the two subunits of hcPheRS are cloned into two different plasmids, the alpha subunit, residues 1-508, into the vector pET21b+, the beta-subunit, residues 1-589, into pET28b+, for expression in Escherichia coli BLRDE3 cells, no affinity tag is used | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the structure of human cytoplasmic phenylalanyl-tRNA synthetase is determined to a resolution of 3.3 A | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 247000 | - |
heterotetramer | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95363 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation is followed by four chromatography columns, anion exchange on a DEAE-Sepharose column, a heparin-Sepharose column, concentrating the protein on a TSK hydrophobic interaction column and finally gel filtration on a column of superfine Sephadex G-200 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Homo sapiens | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | two alpha and two beta subunits | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytoplasmic phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| cytosolic phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| hcPheRS | - |
Homo sapiens |
| Phenylalanyl-tRNA synthetase | - |
Homo sapiens |
| PheRS | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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