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Literature summary for 6.1.1.21 extracted from
- Purification and some properties of the histidyl-tRNA synthetase from cytosol of rabbit reticulocytes (1978), Biochemistry, 17, 1509-1514.
General Stability
| General Stability | Organism |
|---|---|
| no stabilization by glycerol or His, ATP or unfractionated tRNA at concentrations similar to those in the enzyme assay | Oryctolagus cuniculus |
| stability is reduced at protein concentrations of less than 0.015 mg/ml | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-hydroxymercuribenzoate | - |
Oryctolagus cuniculus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000004 | - |
tRNAHisII | substrate from rabbit reticuloytes | Oryctolagus cuniculus | |
| 0.000006 | - |
tRNAHis | unfractionated substrate from rabbit liver or rabbit reticulocyte tRNAHisI | Oryctolagus cuniculus | |
| 0.0005 | - |
L-His | - |
Oryctolagus cuniculus | |
| 0.08 | - |
His | ATP | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration is 45 mM in absence of KCl, 5 mM in presence of 160 mM KCl | Oryctolagus cuniculus | |
| Mg2+ | optimal concentration is 45 mM in absence of KCl, 5 mM in presence of 160 mM KCl | Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
2 * 64000, SDS-PAGE | Oryctolagus cuniculus |
| 122000 | - |
sucrose density gradient centrifugation | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| reticulocyte | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.687 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -85°C, stable for many months | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-histidine + tRNAHis | - |
Oryctolagus cuniculus | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 64000, SDS-PAGE | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9 | enzyme activity does not vary much in this range | Oryctolagus cuniculus |
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