Cloned (Comment) | Organism |
---|---|
DNA sequence determination and expression in strain JM105 of wild-type and mutant enzymes | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Q118E | site-directed point mutagenesis of an active site residue, reduced kcat but unaltered Km with wild-type tRNAHis G-1 | Escherichia coli |
R116A | site-directed mutagenesis, reduced activity, increased specificity for the wild-type full length tRNAHis G-1 | Escherichia coli |
R123A | site-directed point mutagenesis of an active site residue, reduced activity, increased specificity for the wild-type full length tRNAHis G-1 | Escherichia coli |
R9H | site-directed point mutangenesis, increased activity with wild-type full length tRNAHis G-1, reduced activity with the synthetic tRNAHis G-1 and A-1 substrates, increased specificity for the wild-type full length tRNAHis G-1 | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and substrate specificity | Escherichia coli | |
0.00034 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.00096 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.0012 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.0025 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.0026 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.0048 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.0055 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
0.0058 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
0.0063 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
0.0081 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
0.015 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
0.0178 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + tRNAHis | Escherichia coli | - |
AMP + diphosphate + L-histidyl-tRNAHis | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutants | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-histidine + tRNAHis = AMP + diphosphate + L-histidyl-tRNAHis | interaction mechanism of RNA substrate and enzyme, active site structure | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-histidine + synthetic tRNAHis A-1 | - |
Escherichia coli | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
ATP + L-histidine + synthetic tRNAHis G-1 | - |
Escherichia coli | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
ATP + L-histidine + tRNAHis | - |
Escherichia coli | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
ATP + L-histidine + wild-type full length tRNAHis G-1 | activity is highly dependent upon the recognition of the unique G-1:C73 base pair and the 5'-monophosphate | Escherichia coli | AMP + diphosphate + L-histidyl-tRNAHis | - |
? | |
additional information | substrate specificity for the N-1 base of wild-type enzyme and mutants, overview | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HisRS | - |
Escherichia coli |
Histidine translase | - |
Escherichia coli |
histidine-tRNA ligase | - |
Escherichia coli |
histidine-tRNA ligase homolog | - |
Escherichia coli |
Histidyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Histidyl-tRNA synthetase | - |
Escherichia coli |
Jo-1 | - |
Escherichia coli |
Jo-1 antigen | - |
Escherichia coli |
Synthetase, histidyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.012 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
0.018 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
0.024 | - |
synthetic tRNAHis A-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
0.11 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.12 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R123A | Escherichia coli | |
0.13 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
0.76 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
1.76 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
2.04 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
2.5 | 4 | wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
2.54 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli | |
2.94 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, enzyme mutant R116A | Escherichia coli | |
2.94 | - |
wild-type full length tRNAHis G-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
4.75 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, wild-type enzyme | Escherichia coli | |
6.08 | - |
synthetic tRNAHis G-1 | 7.5, 37°C, enzyme mutant R9H | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |