Protein Variants | Comment | Organism |
---|---|---|
Y515A | 109% of wild-type activity | Giardia intestinalis |
Y515E | 42% of wild-type activity | Giardia intestinalis |
Y515K | 51% of wild-type activity | Giardia intestinalis |
Y520A | 68% of wild-type activity | Giardia intestinalis |
Y520E | 41% of wild-type activity | Giardia intestinalis |
Y520H | 46% of wild-type activity | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0054 | - |
L-leucine | mutant Y515A, pH 8.2, 45°C | Giardia intestinalis | |
0.0054 | - |
L-leucine | mutant Y515E, pH 8.2, 45°C | Giardia intestinalis | |
0.0054 | - |
L-leucine | mutant Y520H, pH 8.2, 45°C | Giardia intestinalis | |
0.006 | - |
L-leucine | mutant Y520A, pH 8.2, 45°C | Giardia intestinalis | |
0.0061 | - |
L-leucine | wild-type, pH 8.2, 45°C | Giardia intestinalis | |
0.0077 | - |
L-leucine | mutant Y520E, pH 8.2, 45°C | Giardia intestinalis | |
0.008 | - |
L-leucine | mutant Y515K, pH 8.2, 45°C | Giardia intestinalis | |
0.0092 | - |
L-isoleucyl-tRNALeu | wild-type, pH 7.5, 37°C | Giardia intestinalis | |
0.0122 | - |
L-isoleucyl-tRNALeu | mutant Y515A, pH 7.5, 37°C | Giardia intestinalis | |
0.0147 | - |
L-isoleucyl-tRNALeu | mutant Y520H, pH 7.5, 37°C | Giardia intestinalis | |
0.0173 | - |
L-isoleucyl-tRNALeu | mutant Y520A, pH 7.5, 37°C | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Giardia intestinalis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
L-isoleucyl-tRNALeu + H2O | - |
Giardia intestinalis | t-RNALeu + isoleucine | editing activity | ? | |
additional information | residues Y515 and Y520 outside the editing active site of CP1 domain of Giardia lamblia LeuRS are crucial for post-transfer editing by influencing the binding affinity with mischarged tRNALeu | Giardia intestinalis | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-isoleucyl-tRNALeu | mutant Y515A, pH 7.5, 37°C | Giardia intestinalis | |
0.48 | - |
L-isoleucyl-tRNALeu | mutant Y520A, pH 7.5, 37°C | Giardia intestinalis | |
0.93 | - |
L-isoleucyl-tRNALeu | mutant Y520H, pH 7.5, 37°C | Giardia intestinalis | |
1 | - |
L-leucine | mutant Y515E, pH 8.2, 45°C | Giardia intestinalis | |
1.1 | - |
L-leucine | mutant Y520H, pH 8.2, 45°C | Giardia intestinalis | |
1.4 | - |
L-leucine | mutant Y520E, pH 8.2, 45°C | Giardia intestinalis | |
1.52 | - |
L-isoleucyl-tRNALeu | wild-type, pH 7.5, 37°C | Giardia intestinalis | |
1.8 | - |
L-leucine | mutant Y515K, pH 8.2, 45°C | Giardia intestinalis | |
1.8 | - |
L-leucine | mutant Y520A, pH 8.2, 45°C | Giardia intestinalis | |
2.6 | - |
L-leucine | mutant Y515A, pH 8.2, 45°C | Giardia intestinalis | |
2.7 | - |
L-leucine | wild-type, pH 8.2, 45°C | Giardia intestinalis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-isoleucyl-tRNALeu | mutant Y515A, pH 7.5, 37°C | Giardia intestinalis | |
0.028 | - |
L-isoleucyl-tRNALeu | mutant Y520A, pH 7.5, 37°C | Giardia intestinalis | |
0.063 | - |
L-isoleucyl-tRNALeu | mutant Y520H, pH 7.5, 37°C | Giardia intestinalis | |
0.165 | - |
L-isoleucyl-tRNALeu | wild-type, pH 7.5, 37°C | Giardia intestinalis | |
0.181 | - |
L-leucine | mutant Y520E, pH 8.2, 45°C | Giardia intestinalis | |
0.185 | - |
L-leucine | mutant Y515E, pH 8.2, 45°C | Giardia intestinalis | |
0.203 | - |
L-leucine | mutant Y520H, pH 8.2, 45°C | Giardia intestinalis | |
0.225 | - |
L-leucine | mutant Y515K, pH 8.2, 45°C | Giardia intestinalis | |
0.3 | - |
L-leucine | mutant Y520A, pH 8.2, 45°C | Giardia intestinalis | |
0.442 | - |
L-leucine | wild-type, pH 8.2, 45°C | Giardia intestinalis | |
0.481 | - |
L-leucine | mutant Y515A, pH 8.2, 45°C | Giardia intestinalis |