Protein Variants | Comment | Organism |
---|---|---|
D399A | 40fold increase in Km for leucine activation. Mutation eliminates Ile-tRNALeu deacylation activity | Homo sapiens |
T298A | activity similar to wild-type, mutation maintains Ile-tRNALeu deacylation activity | Homo sapiens |
T298Y | mutation uncouples specificity in the editing active site and mutant hydrolyzes Leu-tRNALeu | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
L-leucine | wild-type, pH 7.6, 30°C | Homo sapiens | |
0.9 | - |
L-leucine | mutant D399A, pH 7.6, 30°C | Homo sapiens | |
2.04 | - |
L-isoleucine | wild-type, pH 7.6, 30°C | Homo sapiens | |
3.3 | - |
L-isoleucine | mutant D399A, pH 7.6, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9P2J5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
L-isoleucyl-tRNALeu + H2O | - |
Homo sapiens | t-RNALeu + L-isoleucine | editing activity | ? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.34 | - |
L-isoleucine | wild-type, pH 7.6, 30°C | Homo sapiens | |
0.51 | - |
L-isoleucine | mutant D399A, pH 7.6, 30°C | Homo sapiens | |
2.1 | - |
L-leucine | wild-type, pH 7.6, 30°C | Homo sapiens | |
5.6 | - |
L-leucine | mutant D399A, pH 7.6, 30°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
L-isoleucine | wild-type, pH 7.6, 30°C | Homo sapiens | |
0.17 | - |
L-isoleucine | mutant D399A, pH 7.6, 30°C | Homo sapiens | |
5.6 | - |
L-leucine | mutant D399A, pH 7.6, 30°C | Homo sapiens | |
94.3 | - |
L-leucine | wild-type, pH 7.6, 30°C | Homo sapiens |