Protein Variants | Comment | Organism |
---|---|---|
W314F | site-directed mutagenesis, activity is similar to the wild-type enzyme | Geobacillus stearothermophilus |
W314f/W332F | site-directed mutagenesis, slightly reduced activity | Geobacillus stearothermophilus |
W332F | site-directed mutagenesis, activity is slightly reduced, but the binding of L-lysine is altered, increased Km for L-lysine | Geobacillus stearothermophilus |
Y271F | site-directed mutagenesis, highly increased Km for L-lysine in the ATP-diphosphate exchange reaction | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
L-lysine | ATP-diphosphate exchange reaction, recombinant wild-type enzyme and mutant W314F/W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.01 | - |
L-lysine | ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.016 | - |
L-lysine | aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.017 | - |
L-lysine | aminoacylation reaction, recombinant mutants W314F and W314F/W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.018 | - |
L-lysine | ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.022 | - |
L-lysine | aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.038 | - |
ATP | aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.043 | - |
ATP | aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.049 | - |
ATP | aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
0.066 | - |
ATP | aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
8.1 | - |
L-lysine | ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | Geobacillus stearothermophilus | - |
AMP + L-lysyl-tRNALys + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
homogenously purified recombinant class II enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys | Trp314 is involved in binding of L-lysine, binding renders the non-polar microenvironment of the residue more polar | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + lysine + tRNALys | - |
Geobacillus stearothermophilus | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
ATP + lysine + tRNALys | two-step reaction mechansim, the first step of formation of the aminoacylated enzyme-AMP intermediate is reversible, the second of amino acid transfer to the tRNA is not, binding of L-lysine alone influences the fluorescence of the enzyme, while binding of ATP does not | Geobacillus stearothermophilus | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Geobacillus stearothermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-Lysine-transfer RNA ligase | - |
Geobacillus stearothermophilus |
Lysine translase | - |
Geobacillus stearothermophilus |
Lysine--tRNA ligase | - |
Geobacillus stearothermophilus |
Lysine-tRNA synthetase | - |
Geobacillus stearothermophilus |
LysRS | - |
Geobacillus stearothermophilus |
Lysyl-transfer ribonucleate synthetase | - |
Geobacillus stearothermophilus |
Lysyl-transfer RNA synthetase | - |
Geobacillus stearothermophilus |
Lysyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
Synthetase, lysyl-transfer ribonucleate | - |
Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3 | 6 | L-lysine | aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.13 | - |
L-lysine | aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.13 | - |
L-lysine | aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.23 | - |
L-lysine | aminoacylation reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.3 | - |
L-lysine | aminoacylation reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.53 | - |
L-lysine | aminoacylation reaction, recombinant wild-type enzyme, pH 8.0, 37°C | Geobacillus stearothermophilus | |
3.58 | - |
L-lysine | aminoacylation reaction, recombinant mutant W314F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
34.7 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mutant Y271F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
36.4 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mutant W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
37.3 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mutant W314F/W332F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
38.6 | - |
ATP | ATP-diphosphate exchange reaction, recombinant mutant W314F, pH 8.0, 37°C | Geobacillus stearothermophilus | |
42.8 | - |
ATP | ATP-diphosphate exchange reaction, recombinant wild-type enzyme, pH 8.0, 37°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Geobacillus stearothermophilus |