Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
L356A | only little effect on activity | Arabidopsis thaliana |
M293P/K320L | effectively uses ferulate as substrate, changes in Km for all substrates | Arabidopsis thaliana |
additional information | domain exchange experiments with gramicidine synthetase from Bacillus brevis | Arabidopsis thaliana |
N256A/M293P/K320L | effectively uses ferulate and cinnamate as substrates, 10fold reduced activity with caffeate | Arabidopsis thaliana |
V355A | strongly reduced activity | Arabidopsis thaliana |
V355A/L356A | strongly reduced activity | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
caffeate | wild-type enzyme | Arabidopsis thaliana | |
0.022 | - |
4-coumarate | M293P/K320L mutant enzyme | Arabidopsis thaliana | |
0.03 | - |
ferulate | M293P/K320L mutant enzyme | Arabidopsis thaliana | |
0.041 | - |
caffeate | M293P/K320L mutant enzyme | Arabidopsis thaliana | |
0.233 | - |
4-coumarate | wild-type enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | Arabidopsis thaliana | plant phenylpropanoid metabolism | AMP + diphosphate + 4-coumaroyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
isoform 4CL2 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-coumarate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + 4-coumarate + CoA | plant phenylpropanoid metabolism | Arabidopsis thaliana | AMP + diphosphate + 4-coumaroyl-CoA | - |
? | |
ATP + caffeate + CoA | - |
Arabidopsis thaliana | AMP + diphosphate + caffeoyl-CoA | - |
? | |
ATP + ferulate + CoA | very poor substrate for the wild type enzyme, effective substrate for M293P/K320L mutant enzyme | Arabidopsis thaliana | AMP + diphosphate + feruloyl-CoA | - |
? |