Protein Variants | Comment | Organism |
---|---|---|
K46E/K114D | the double mutant shows reduced activity with ATP and high specificity towards GTP compared to the wild type enzyme | Blastocystis hominis |
K46E/K114D/V113L/L227F | the quadruple mutant shows no activity with ATP and high activity towards GTP compared to the wild type enzyme | Blastocystis hominis |
V113L/L227F | the double mutant shows increased activity with ATP compared to the wild type enzyme | Blastocystis hominis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
GTP | mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis | |
0.143 | - |
GTP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis | |
0.145 | - |
ATP | wild type enzyme, at pH 7.4 and 21°C | Blastocystis hominis | |
0.23 | - |
ATP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis | |
0.265 | - |
ATP | mutant enzyme V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + succinate + CoA | Blastocystis hominis | - |
ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | Blastocystis hominis NandII | - |
ADP + phosphate + succinyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Blastocystis hominis | - |
- |
- |
Blastocystis hominis NandII | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + succinate + CoA | - |
Blastocystis hominis | ADP + phosphate + succinyl-CoA | - |
? | |
ATP + succinate + CoA | - |
Blastocystis hominis NandII | ADP + phosphate + succinyl-CoA | - |
? | |
GTP + succinate + CoA | substrate for mutant enzymes K46E/K114D and K46E/K114D/V113L/L227F | Blastocystis hominis | GDP + phosphate + succinyl-CoA | - |
? | |
GTP + succinate + CoA | substrate for mutant enzymes K46E/K114D and K46E/K114D/V113L/L227F | Blastocystis hominis NandII | GDP + phosphate + succinyl-CoA | - |
? | |
additional information | the wild type enzyme has no activity with GTP | Blastocystis hominis | ? | - |
? | |
additional information | the wild type enzyme has no activity with GTP | Blastocystis hominis NandII | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Blastocystis hominis |
Synonyms | Comment | Organism |
---|---|---|
ATP-specific SCS | - |
Blastocystis hominis |
SCSalpha | - |
Blastocystis hominis |
Succinyl-CoA synthetase | - |
Blastocystis hominis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
GTP | mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis | |
0.0139 | - |
ATP | wild type enzyme, at pH 7.4 and 21°C | Blastocystis hominis | |
0.0176 | - |
ATP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis | |
0.0177 | - |
ATP | mutant enzyme V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis | |
0.0195 | - |
GTP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Blastocystis hominis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
ATP | mutant enzyme V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis | |
0.076 | - |
ATP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis | |
0.096 | - |
GTP | mutant enzyme K46E/K114D/V113L/L227F, at pH 7.4 and 21°C | Blastocystis hominis | |
0.096 | - |
ATP | wild type enzyme, at pH 7.4 and 21°C | Blastocystis hominis | |
0.136 | - |
GTP | mutant enzyme K46E/K114D, at pH 7.4 and 21°C | Blastocystis hominis |