Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizobium leguminosarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0267 | - |
CoA | pH and temperature not specified in the publication | Rhizobium leguminosarum | |
0.0338 | - |
ATP | pH and temperature not specified in the publication | Rhizobium leguminosarum | |
0.113 | - |
malonate | pH and temperature not specified in the publication | Rhizobium leguminosarum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54612 | - |
calculated from sequence | Rhizobium leguminosarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + malonate + CoA | Rhizobium leguminosarum | - |
AMP + diphosphate + malonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizobium leguminosarum | Q9ZIP5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhizobium leguminosarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.46 | - |
pH and temperature not specified in the publication | Rhizobium leguminosarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-methylmalonate + CoA | activity is 20.4% compared to the activity with malonate | Rhizobium leguminosarum | AMP + diphosphate + 2-methylmalonyl-CoA | - |
? | |
ATP + malonate + CoA | - |
Rhizobium leguminosarum | AMP + diphosphate + malonyl-CoA | - |
? | |
additional information | no activity for acetate, propionate and succinate in place of malonate, and for GTP, CTP and AMP in place of ATP | Rhizobium leguminosarum | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 54612, calculated from sequence | Rhizobium leguminosarum |
Synonyms | Comment | Organism |
---|---|---|
malonyl-CoA synthetase | - |
Rhizobium leguminosarum |
matB | - |
Rhizobium leguminosarum |