Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli DH1 cells | Acinetobacter calcoaceticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
ATP | 30°C, pH 8.2, for the L-aspartic acid-ATP binding site alone | Acinetobacter calcoaceticus | |
0.24 | - |
L-aspartic acid | 30°C, pH 8.2 | Acinetobacter calcoaceticus | |
0.278 | - |
ATP | 30°C, pH 8.2, for the complete reaction | Acinetobacter calcoaceticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Acinetobacter calcoaceticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | Acinetobacter calcoaceticus | - |
ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
additional information | Acinetobacter calcoaceticus | without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion-exchange chromatography, gel filtration, affinity precipitation with cyanophycin, 69fold purification | Acinetobacter calcoaceticus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by the enzyme's other active centre | Acinetobacter calcoaceticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.265 | - |
30°C, pH 8.2 | Acinetobacter calcoaceticus |
Storage Stability | Organism |
---|---|
at 7°C the purified enzyme is stable for about one week, thereafter the activity decreases rapidly, storage at -20°C in the presence of 10% (w/v) DMSO or 50% (w/v) glycerol does not improve the stability significantly | Acinetobacter calcoaceticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | - |
Acinetobacter calcoaceticus | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
L-aspartic acid + ATP | - |
Acinetobacter calcoaceticus | poly-L-aspartic acid + ADP + phosphate | - |
? | |
additional information | without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis | Acinetobacter calcoaceticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cyanophycin synthetase | - |
Acinetobacter calcoaceticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
30°C | Acinetobacter calcoaceticus |