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Literature summary for 6.3.4.20 extracted from
- The nitrile-forming enzyme 7-cyano-7-deazaguanine synthase from Geobacillus kaustophilus A reverse nitrilase? (2015), ChemBioChem, 16, 2373-2378 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed at high level in Escherichia coli, and the protein is expressed with an N-terminal His tag-target protein | Geobacillus kaustophilus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| molecular modeling of QueC-AMP complex. Residues Q39, T119, R97, and N98 are most likely involved in ATP binding. CDG binding likely involves residues Y129 or Y187 for pi-stacking of the CDG substrate, and D125 or D131 in binding the primary and secondary amines of the substrate pyrimidine | Geobacillus kaustophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | the enzyme harbors Zn2+ and Mg2+ ions | Geobacillus kaustophilus |  |
| Zn2+ | Zn2+ ion seems to be of mainly structural importance | Geobacillus kaustophilus | |
| Zn2+ | the enzyme harbors Zn2+ and Mg2+ ions, coordinated by a cysteine-rich binding motive | Geobacillus kaustophilus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus | - |
7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus DSM-7263 | - |
7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus DSM-7263 | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus NBRC 102445 | - |
7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | Geobacillus kaustophilus NBRC 102445 | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus kaustophilus | A0A0N9HMW6 | - |
- |
| Geobacillus kaustophilus DSM-7263 | A0A0N9HMW6 | - |
- |
| Geobacillus kaustophilus NBRC 102445 | A0A0N9HMW6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus kaustophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-carboxy-7-carbaguanine + NH3 + ATP | - |
Geobacillus kaustophilus | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | Geobacillus kaustophilus | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | strict substrate specificity for 7-carboxy-7-carbaguanine. No ADP or phosphate is detected as product | Geobacillus kaustophilus | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | - |
Geobacillus kaustophilus DSM-7263 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | Geobacillus kaustophilus DSM-7263 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | strict substrate specificity for 7-carboxy-7-carbaguanine. No ADP or phosphate is detected as product | Geobacillus kaustophilus DSM-7263 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | - |
Geobacillus kaustophilus NBRC 102445 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | Geobacillus kaustophilus NBRC 102445 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| 7-carboxy-7-carbaguanine + NH3 + ATP | strict substrate specificity for 7-carboxy-7-carbaguanine. No ADP or phosphate is detected as product | Geobacillus kaustophilus NBRC 102445 | 7-cyano-7-carbaguanine + AMP + diphosphate + H2O | - |
? | |
| additional information | no substrates: indole-3-carboxylic acid, cinnamic acid, pyrrole-2-carboxylic acid, benzoic acid, pyridine-2-carboxylic acid, 3-phenylpropionic acid, 2-methyl-4-oxo-4,5,6,7-tetrahydro-1H-indole-3-carboxylic acid, pyrrole-3-carboxylic acid, rac-mandelic acid, pyrazinecarboxylic acid | Geobacillus kaustophilus | ? | - |
? | |
| additional information | no substrates: indole-3-carboxylic acid, cinnamic acid, pyrrole-2-carboxylic acid, benzoic acid, pyridine-2-carboxylic acid, 3-phenylpropionic acid, 2-methyl-4-oxo-4,5,6,7-tetrahydro-1H-indole-3-carboxylic acid, pyrrole-3-carboxylic acid, rac-mandelic acid, pyrazinecarboxylic acid | Geobacillus kaustophilus DSM-7263 | ? | - |
? | |
| additional information | no substrates: indole-3-carboxylic acid, cinnamic acid, pyrrole-2-carboxylic acid, benzoic acid, pyridine-2-carboxylic acid, 3-phenylpropionic acid, 2-methyl-4-oxo-4,5,6,7-tetrahydro-1H-indole-3-carboxylic acid, pyrrole-3-carboxylic acid, rac-mandelic acid, pyrazinecarboxylic acid | Geobacillus kaustophilus NBRC 102445 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| queC | - |
Geobacillus kaustophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Geobacillus kaustophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75.5 | - |
melting temperature without substrate | Geobacillus kaustophilus |
| 78.5 | - |
melting temperature in presence of 7-carboxy-7-carbaguanine | Geobacillus kaustophilus |
| 81 | - |
melting temperature in presence of ATP | Geobacillus kaustophilus |
| 82 | - |
melting temperature, in the presence of ATP and substrate | Geobacillus kaustophilus |
| 82 | - |
melting temperature in presence of ATP and 7-carboxy-7-carbaguanine | Geobacillus kaustophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Geobacillus kaustophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | pH 8.0: about 70% of maximal activity, pH 10.0: about 70% of maximal activity | Geobacillus kaustophilus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in biosynthetic pathways towards deazapurine-containing compounds | Geobacillus kaustophilus |
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Release 2023.1 (January 2023)
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