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Literature summary for 6.4.1.2 extracted from

  • Ahmad, F.; Ahmad, P.M.
    Acetyl-CoA carboxylase from rat mammary gland (including an additional step yielding fatty acid synthase) (1981), Methods Enzymol., 71, 16-26.
    View publication on PubMed
Search for more literature for 6.4.1.2

Activating Compound

Activating Compound Comment Organism Structure
citrate maximal activation at 25-30 mM Rattus norvegicus
citrate completely inactive in absence of the allosteric activator citrate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
4-bromo-2,3-dioxobutyl-CoA
-
 Rattus norvegicus
p-chloromercuribenzoate
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
phosphate contains approximately 6 mol phosphate per 240000 g of protein Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.2
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
20°C, 50 mM imidazole HCl, 0.1 mM EDTA, 0.02% sodium azide, 20% glycerol, 25 mM citrate, pH 7.5, stable for up to 1 week Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetyl-CoA + HCO3-
-
 Rattus norvegicus ADP + phosphate + malonyl-CoA
-
 ?

Subunits

Subunits Comment Organism
? x * 240000-260000, SDS-PAGE of enzyme dissociated in 1% SDS, 8 M urea and 5 mM DTT Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
biotin contains 1 mol biotin per 240000 g of enzyme Rattus norvegicus