Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NADP+, NADPH, 3-hydroxypropionate, succinate, fumarate, malate, and glyoxylate have no significant effect on acetyl-CoA carboxylase or propionyl-CoA carboxylase activities | Acidianus brierleyi | |
palmitoyl-CoA | 0.5 mM palmitoyl-CoA has a slight stimulative effect on acetyl-CoA carboxylase (116%) and propionyl-CoA carboxylase (135%) activities | Acidianus brierleyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | an excess of ATP (2.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
citrate | citrate inhibits the enzyme activity by more than 50% at concentrations of 3 mM | Acidianus brierleyi | |
Co2+ | 26% residual activity at 10-15 mM | Acidianus brierleyi | |
CoASH | inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% | Acidianus brierleyi | |
dithiothreitol | dithiothreitol inhibits the enzyme slightly, by approximately 12%, at a final concentration of 5 to 10 mM | Acidianus brierleyi | |
K+ | the enzyme shows less than 50% activity when the concentration is higher than 40 mM | Acidianus brierleyi | |
malonyl-CoA | inhibits acetyl-CoA carboxylase activity 50 to 57% and propionyl-CoA carboxylase activity 11 to 13% | Acidianus brierleyi | |
methylmalonyl-CoA | 60% inhibition at 0.5 mM; 79% inhibition at 0.5 mM | Acidianus brierleyi | |
Mg2+ | an excess of Mg2+ (higher than 4.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
Mn2+ | an excess of Mn2+ (higher than 2.0 mM) has an inhibitory effect on the enzymatic activities | Acidianus brierleyi | |
Na+ | the enzyme shows less than 50% activity when the concentration is higher than 40 mM | Acidianus brierleyi | |
succinyl-CoA | 57% inhibition at 0.5 mM; 84% inhibition at 0.5 mM | Acidianus brierleyi | |
sulfate | sulfate inhibits the enzyme activity by more than 50% at concentrations of 25 mM | Acidianus brierleyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
ATP | with acetyl-CoA as cosubstrate, at pH 7.5 and 70°C | Acidianus brierleyi | |
0.05 | - |
ATP | with propionyl-CoA as cosubstrate, at pH 7.5 and 70°C | Acidianus brierleyi | |
0.1 | - |
propionyl-CoA | at pH 7.5 and 70°C | Acidianus brierleyi | |
0.17 | - |
acetyl-CoA | at pH 7.5 and 70°C | Acidianus brierleyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, maximum activity at 4 mM | Acidianus brierleyi | |
additional information | Fe2+, Cu2+, Ca2+, and Ni2+ (0.5 to 20 mM) have no effect on enzyme activity | Acidianus brierleyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
540000 | - |
gel filtration | Acidianus brierleyi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | Acidianus brierleyi | - |
ADP + phosphate + malonyl-CoA | - |
? | |
ATP + propionyl-CoA + HCO3- | Acidianus brierleyi | - |
ADP + phosphate + methylmalonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus brierleyi | Q877I4 and Q877I5 and Q877I3 | - |
- |
Purification (Comment) | Organism |
---|---|
Streptavidin column chromatography and Superose 6 gel filtration | Acidianus brierleyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + acetyl-CoA + HCO3- | - |
Acidianus brierleyi | ADP + phosphate + malonyl-CoA | - |
? | |
ATP + propionyl-CoA + HCO3- | - |
Acidianus brierleyi | ADP + phosphate + methylmalonyl-CoA | - |
? | |
additional information | neither succinyl-CoA, palmitoyl-CoA, acetate (plus 0.5 mM CoASH), propionate (plus 0.5 mM CoASH), nor pyruvate is carboxylated by the enzyme | Acidianus brierleyi | ? | - |
? | |
n-butyryl-CoA + HCO3- | 4.6% activity compared to acetyl-CoA or propionyl-CoA | Acidianus brierleyi | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 4 * 62000 + 4 * 59000 + 4 * 20000, SDS-PAGE | Acidianus brierleyi |
Synonyms | Comment | Organism |
---|---|---|
ACCB | - |
Acidianus brierleyi |
AccC | - |
Acidianus brierleyi |
acyl coenzyme A carboxylase | - |
Acidianus brierleyi |
acyl-CoA carboxylase | - |
Acidianus brierleyi |
PccB | - |
Acidianus brierleyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | - |
Acidianus brierleyi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 90 | the activity of the enzyme gradually decreases at 50°C, with a residual activity of around 60% after 2 h of incubation. At 60°C, the residual activity of the enzyme is 50% after incubation for 35 to 40 min, whereas the residual activities at 70 and 80°C are less than 15%. The enzyme completely loses its activity within 10 min when it is incubated at 90°C or above | Acidianus brierleyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.9 | - |
Acidianus brierleyi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | the enzyme exhibits the maximum activity when the ATP concentration is 1.0-1.5 mM. Neither ADP, AMP, GTP, CTP, UTP, nor dTTP (0.2 mM) can substitute for ATP | Acidianus brierleyi |