Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cations stimulate activity, maximal activation at 10-30 mM | Thermococcus kodakarensis | |
Mg2+ | required for activity, maximal activity at 14-18 mM | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52100 | - |
gel filtration | Thermococcus kodakarensis |
64079 | - |
1 * 64079, deduced from nucleotide sequence | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Thermococcus kodakarensis | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q9HHC4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant ligTK, ResourceQ, ResourceS, Superdex-200 | Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)30 + (deoxyribonucleotide)40 | ligTK displays little but significant ativity with NAD+ | Thermococcus kodakarensis | AMP + diphosphate + (deoxyribonucleotide)70 | - |
? | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Thermococcus kodakarensis | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64079, deduced from nucleotide sequence | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
ligTK | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
at 0.00002 mM ligTK | Thermococcus kodakarensis |
70 | 80 | at 0.0002 mM ligTK | Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | at 0.0002 mM ligTK | Thermococcus kodakarensis |
35 | 80 | at 0.00002 mM ligTK | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermococcus kodakarensis |