Activating Compound | Comment | Organism | Structure |
---|---|---|---|
proliferating cell nuclear antigen | stimulatory effect of Pyrococcus furiosus proliferating cell nuclear antigen (PCNA) on the enzyme activity of Pyrococcus furiosus DNA ligase is observed not at low ionic strength, but at a high salt concentration, at which a DNA ligase alone cannot bind to a nicked DNA substrate. Identification of the amino acid residues that are critical for PCNA binding in a loop structure located in the N-terminal DNA-binding domain of Ppyrococcus furiosus DNA ligase. The pentapeptide motif QKSFF is involved in the PCNA-interacting motifs, in which Gln and the first Phe are especially important for stable binding with PCNA | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
K249A | mutant enzyme shows no no adenylyltransferase activity | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | P56709 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | no DNA ligase activity in presence of ADP, AMP, and NAD+ | Pyrococcus furiosus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PfuLig | - |
Pyrococcus furiosus |