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Literature summary for 7.1.1.7 extracted from

  • Yang, K.; Zhang, J.; Vakkasoglu, A.S.; Hielscher, R.; Osborne, J.P.; Hemp, J.; Miyoshi, H.; Hellwig, P.; Gennis, R.B.
    Glutamate 107 in subunit I of the cytochrome bd quinol oxidase from Escherichia coli is protonated and near the heme d/heme b595 binuclear center (2007), Biochemistry, 46, 3270-3278.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D239A mutation in subunit cydA. Mutant is not able to confer the ability to grow aerobically to a strain that has no genomically encoded respiratory oxidases. Heme content similar to wild-type, complete loss of ubiquinol oxidase activity Escherichia coli
D239N mutation in subunit cydA. Mutant is not able to confer the ability to grow aerobically to a strain that has no genomically encoded respiratory oxidases. Heme content similar to wild-type, complete loss of ubiquinol oxidase activity Escherichia coli
D29E mutation in subunit cydB. Mutant is totally inactive, and hemes d and b595 are not present in the isolated enzyme Escherichia coli
E107D mutation in subunit cydA. About 15% of the heme contant of wild-type, complete loss of ubiquinol oxidase activity Escherichia coli
E107Q mutation in transmembrane helix III of subunit cydA. Mutant is totally inactive, but retains its hemes. Residue E107 is protonated at pH 7.6 and is perturbed by the reduction of the heme d/heme b595 binuclear center at the active site Escherichia coli
E257A mutation of an acidic residue of subunit cydA at or near the quinol-binding site, mutation inactivates the enzyme but has no substantial influence on the Fourier transform infrared redox difference spectrum Escherichia coli
E99A mutation in subunit cydA. Mutant is not able to confer the ability to grow aerobically to a strain that has no genomically encoded respiratory oxidases. Complete loss of heme d and heme b595 and of ubiquinol oxidase activity Escherichia coli
E99Q mutation in subunit cydA. Mutant is not able to confer the ability to grow aerobically to a strain that has no genomically encoded respiratory oxidases. Complete loss of heme d and heme b595 and of ubiquinol oxidase activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABJ9 subunit cydA
-
Escherichia coli P0ABK2 subunit cydB
-