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Literature summary for 7.1.1.9 extracted from
- Characterization and application of a thermostable primary transport system: cytochrome c oxidase from Bacillus stearothermophilus (1989), Eur. J. Biochem., 178, 763-770.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | 0.08 mM, 50% inhibition | Geobacillus stearothermophilus |  |
| CN- | 0.0001 mM, 50% inhibition | Geobacillus stearothermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu | - |
Geobacillus stearothermophilus | |
| Fe | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Geobacillus stearothermophilus |
| 37000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Geobacillus stearothermophilus |
| 57000 | - |
1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| large scale | Geobacillus stearothermophilus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| reconstitution | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ferrocytochrome c + O2 | Saccharomyces cerevisiae cytochrome c | Geobacillus stearothermophilus | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | horse ferrocytochrome c | Geobacillus stearothermophilus | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | artificial electron donor: phenazine methosulfate | Geobacillus stearothermophilus | ferricytochrome c + H2O | - |
? | |
| ferrocytochrome c + O2 | artificial electron donor: ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine | Geobacillus stearothermophilus | ferricytochrome c + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 1 * 57000 + 1 * 37000 + 1 * 22000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 81 | - |
inactivation, no protection by phospholipids | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
proton translocation | Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | proton translocation | Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme a | - |
Geobacillus stearothermophilus | |
| Heme a3 | - |
Geobacillus stearothermophilus | |
| heme c | firmly bound | Geobacillus stearothermophilus | |
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Release 2023.1 (January 2023)
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