Crystallization (Comment) | Organism |
---|---|
orthorhombic crystals of F1-ATPase, grown during 4 weeks from 5 mg/ml protein solution mixed with an equal volume of crystallization solution containing 50 mM Tris-HCl, pH 8.0, 200 mM NaCl, 20 mM MgSO4, 0.02% w/v NaN3, 0.004% w/v PMSF, 1 mM ATP and 9-12% w/v PEG 6000 in D2O, are subjected to controlled dehydration resulting in a decrease in relative humidity surrounding the crystals to 90%, in reduced unit-cell volume by 22%, and significantly improved diffraction limit and mosaic spread of the crystals, X-ray diffraction and structure analysis at 1.8 A resolution, radiation damage limits the resolution of a complete data set to 1.95 A, overview | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial membrane | - |
Bos taurus | 31966 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Bos taurus | F1Fo-ATPase is a large membrane-bound multisubunit complex that catalyses the synthesis of ATP from ADP and phosphate using a transmembrane proton motive force generated by respiration or photosynthesis as a source of energy, ATP hydrolytic catalysis takes place in its hydrophilic F1 domain | ADP + phosphate + H+/out | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bos taurus | ADP + phosphate + H+/out | - |
? | |
ATP + H2O + H+/in | F1Fo-ATPase is a large membrane-bound multisubunit complex that catalyses the synthesis of ATP from ADP and phosphate using a transmembrane proton motive force generated by respiration or photosynthesis as a source of energy, ATP hydrolytic catalysis takes place in its hydrophilic F1 domain | Bos taurus | ADP + phosphate + H+/out | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
mitochondrial F(1)-ATPase | - |
Bos taurus |