Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.3.1 extracted from

  • Masuda, H.; Uchiumi, T.; Wada, M.; Ichiba, T.; Hachimori, A.
    Effects of replacement of prolines with alanines on the catalytic activity and thermostability of inorganic pyrophosphatase from thermophilic bacterium PS-3 (2002), J. Biochem., 131, 53-58.
    View publication on PubMed
Search for more literature for 7.1.3.1

Cloned(Commentary)

Cloned (Comment) Organism
wild type and mutant enzymes expressed in Escherichia coli JM109 Bacillus sp. PS3

Protein Variants

Protein Variants Comment Organism
P100A activity almost completely abolished, trimer, heat labile Bacillus sp. PS3
P104A activity almost completely abolished, trimer, heat labile Bacillus sp. PS3
P116A large decrease in activity, almost fully active at pH 10 Bacillus sp. PS3
P146A activity almost completely abolished, trimer, heat labile Bacillus sp. PS3
P14A large decrease in activity, formation of trimers during heat treatment Bacillus sp. PS3
P39A slight increase in activity, almost fully active at pH 10 Bacillus sp. PS3
P43A large decrease in activity, formation of trimers during heat treatment Bacillus sp. PS3
P59A large decrease in activity, narrow pH optimum at pH 8 Bacillus sp. PS3
P69A slight decrease in activity Bacillus sp. PS3
P72A activity completely abolished, monomer Bacillus sp. PS3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 diphosphate P69A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.47
-
 diphosphate wild type enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.55
-
 diphosphate P146A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.6
-
 diphosphate P39A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.64
-
 diphosphate P116A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.82
-
 diphosphate P14A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.88
-
 diphosphate P100A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
0.96
-
 diphosphate P59A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
1
-
 diphosphate P104A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
1
-
 diphosphate P43A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + H2O Bacillus sp. PS3 important for energy metabolism, provides energy for biosynthetic reactions 2 phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus sp. PS3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + H2O
-
 Bacillus sp. PS3 2 phosphate
-
 ?
diphosphate + H2O important for energy metabolism, provides energy for biosynthetic reactions Bacillus sp. PS3 2 phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer composed of two trimers, hexameric form required for full activity and thermostability, gel filtration Bacillus sp. PS3
trimer in the absence of Mg2+ Bacillus sp. PS3

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 diphosphate P104A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
2.8
-
 diphosphate P100A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
19
-
 diphosphate P146A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
74.3
-
 diphosphate P14A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
236.7
-
 diphosphate P116A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
288.7
-
 diphosphate P43A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
413.3
-
 diphosphate P59A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
744.3
-
 diphosphate P69A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3
817
-
 diphosphate wild type enzyme, pH 8.0, 37°C Bacillus sp. PS3
1098
-
 diphosphate P39A mutant enzyme, pH 8.0, 37°C Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9
-
 Bacillus sp. PS3