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Literature summary extracted from

  • Pongsamart, S.; Ho, R.I.; Corman, L.; Foye, W.O.
    Characterization and inhibition of dihydrofolate synthetase from Neisseria gonorrhoeae (1984), Mol. Cell. Biochem., 59, 165-171.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.12 7,8-dihydrofolate noncompetitive with respect to dihydropteroate, competitive with respect to L-Glu Neisseria gonorrhoeae
6.3.2.12 p-hydroxymercuribenzoate
-
 Neisseria gonorrhoeae
6.3.2.12 PCMB
-
 Neisseria gonorrhoeae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.12 0.035
-
 7,8-dihydropteroate
-
 Neisseria gonorrhoeae
6.3.2.12 0.65
-
 L-Glu
-
 Neisseria gonorrhoeae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.12 K+ maximal activity at 80 mM Neisseria gonorrhoeae
6.3.2.12 K+ monovalent cation requirement is satisfied by K+ or NH4+ Neisseria gonorrhoeae
6.3.2.12 Mg2+ divalent cation required, Mg2+ or Mn2+ Neisseria gonorrhoeae
6.3.2.12 Mn2+ divalent cation required Neisseria gonorrhoeae
6.3.2.12 NH4+ monovalent cation required, K+ or NH4+, maximal activity at 20 mM Neisseria gonorrhoeae

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Neisseria gonorrhoeae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.12 additional information
-
-
 Neisseria gonorrhoeae

Storage Stability

EC Number Storage Stability Organism
6.3.2.12 -60°C, stable Neisseria gonorrhoeae
6.3.2.12 4°C, 50% loss of activity after 6 weeks Neisseria gonorrhoeae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Neisseria gonorrhoeae ADP + phosphate + 7,8-dihydrofolate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.12 9.3
-
-
 Neisseria gonorrhoeae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.12 8 9.5 8: about 25% of maximal activity, 9.5: about 85% of maximal activity Neisseria gonorrhoeae