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Literature summary extracted from
- Purification and characterization of a benzylviologen-linked tungsten-containing aldehyde oxidoreductase from Desulfovibrio gigas (1995), J. Bacteriol., 177, 6195-6200.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.99.6 | AsO2- | - |
Megalodesulfovibrio gigas |  |
| 1.2.99.6 | CN- | - |
Megalodesulfovibrio gigas | |
| 1.2.99.6 | iodoacetate | - |
Megalodesulfovibrio gigas | |
| 1.2.99.6 | methanol | - |
Megalodesulfovibrio gigas | |
| 1.2.99.7 | arsenite | 5 mM, complete inhibition | Megalodesulfovibrio gigas | |
| 1.2.99.7 | iodoacetate | 5 mM, complete inhibition | Megalodesulfovibrio gigas | |
| 1.2.99.7 | KCN | 5 mM, 64% inhibition | Megalodesulfovibrio gigas | |
| 1.2.99.7 | methanol | 1 M, 62% inhibition | Megalodesulfovibrio gigas | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.99.6 | 0.0108 | - |
propanal | with benzyl viologen as cosubstrate | Megalodesulfovibrio gigas | |
| 1.2.99.6 | 0.0125 | - |
acetaldehyde | with benzyl viologen as cosubstrate | Megalodesulfovibrio gigas | |
| 1.2.99.6 | 0.02 | - |
benzaldehyde | with benzyl viologen as cosubstrate | Megalodesulfovibrio gigas | |
| 1.2.99.6 | 0.0308 | - |
furfural | with benzyl viologen as cosubstrate | Megalodesulfovibrio gigas | |
| 1.2.99.6 | 0.55 | - |
benzyl viologen | - |
Megalodesulfovibrio gigas | |
| 1.2.99.7 | 0.55 | - |
benzylviologen | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 1.2.99.7 | 10.8 | - |
propionaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 1.2.99.7 | 12.5 | - |
acetaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 1.2.99.7 | 20 | - |
benzaldehyde | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
| 1.2.99.7 | 30.8 | - |
furfural | 30°C, pH 7.5 | Megalodesulfovibrio gigas | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.99.6 | Iron | contains 4.8 gatom of Fe per mol of subunit | Megalodesulfovibrio gigas | |
| 1.2.99.6 | Iron | possibly contains a [4Fe-4S]1+cluster | Megalodesulfovibrio gigas | |
| 1.2.99.6 | K+ | stimulates, optimal concentration: 10 mM | Megalodesulfovibrio gigas | |
| 1.2.99.6 | Tungsten | enzyme contains 0.68 gatom of tungsten per mol of subunit | Megalodesulfovibrio gigas | |
| 1.2.99.7 | Iron | 4.8 iron per subunit, probably a [Fe-4S]1+ cluster | Megalodesulfovibrio gigas | |
| 1.2.99.7 | K+ | 10 mM, 50% activation | Megalodesulfovibrio gigas | |
| 1.2.99.7 | Tungsten | 0.68 tungsten per subunit | Megalodesulfovibrio gigas | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.6 | 62000 | - |
2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
| 1.2.99.6 | 120000 | 126000 | gel filtration | Megalodesulfovibrio gigas |
| 1.2.99.7 | 62000 | - |
2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
| 1.2.99.7 | 120000 | - |
gelfiltration, Sephacryl S-300 | Megalodesulfovibrio gigas |
| 1.2.99.7 | 126000 | - |
gel filtration, Superose 12 HR | Megalodesulfovibrio gigas |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.99.6 | Megalodesulfovibrio gigas | - |
- |
- |
| 1.2.99.6 | Megalodesulfovibrio gigas NCIMB 9332 | - |
- |
- |
| 1.2.99.7 | Megalodesulfovibrio gigas | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.2.99.6 | unstable to air | Megalodesulfovibrio gigas |
| 1.2.99.7 | exposure to air leads to inactivation | Megalodesulfovibrio gigas |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.99.6 | - |
Megalodesulfovibrio gigas |
| 1.2.99.7 | Q-Sepharose, Butyl-Sepharose, DEAE-memsep 1010 | Megalodesulfovibrio gigas |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.6 | 38.5 | - |
- |
Megalodesulfovibrio gigas |
| 1.2.99.7 | 38.5 | - |
- |
Megalodesulfovibrio gigas |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.99.6 | 4-Methoxybenzaldehyde + benzyl viologen | at 19% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | 4-Methoxybenzoate + reduced carbamoyl methyl viologen | - |
? | |
| 1.2.99.6 | Acetaldehyde + 2,6-dichlorophenol-indophenol | at 1.3% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas | acetate + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.2.99.6 | Acetaldehyde + 2,6-dichlorophenol-indophenol | at 1.3% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas NCIMB 9332 | acetate + reduced 2,6-dichlorophenol-indophenol | - |
? | |
| 1.2.99.6 | Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide | at 5% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas | acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide | - |
? | |
| 1.2.99.6 | Acetaldehyde + 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide | at 5% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas NCIMB 9332 | acetate + reduced 3-(4',5'-dimethylthiazol-2-yl)-2,4-diphenyltetrazolium bromide | - |
? | |
| 1.2.99.6 | Acetaldehyde + benzyl viologen | - |
Megalodesulfovibrio gigas | acetate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Acetaldehyde + benzyl viologen | - |
Megalodesulfovibrio gigas NCIMB 9332 | acetate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Acetaldehyde + methyl viologen | at 19% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas | acetate + reduced methyl viologen | - |
? | |
| 1.2.99.6 | Acetaldehyde + methyl viologen | at 19% of the activity relative to benzyl viologen | Megalodesulfovibrio gigas NCIMB 9332 | acetate + reduced methyl viologen | - |
? | |
| 1.2.99.6 | Benzaldehyde + benzyl viologen | at 93% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Benzoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Benzaldehyde + benzyl viologen | at 93% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas NCIMB 9332 | Benzoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Butyraldehyde + benzyl viologen | at 16% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Butanoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Furfural + benzyl viologen | at 77% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | 2-Furancarboxylic acid + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Glutaraldehyde + benzyl viologen | at 16% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Glutarate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Glycolaldehyde + benzyl viologen | at 23% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Glycolate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Hexanal + benzyl viologen | at 6% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Hexanoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Methanal + benzyl viologen | at 16% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Formate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Pentanal + benzyl viologen | at 9% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Pentanoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Phenylacetaldehyde + benzyl viologen | at 31% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Phenylacetate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Propanal + benzyl viologen | at 117% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Propanoate + reduced benzyl viologen | - |
? | |
| 1.2.99.6 | Salicylaldehyde + benzyl viologen | at 14% of the activity relative to acetaldehyde | Megalodesulfovibrio gigas | Salicylate + reduced benzyl viologen | - |
? | |
| 1.2.99.7 | acetaldehyde + H2O + benzylviologen | natural electron aceptor not yet known, the artificial electron acceptors benzylviologen, methylviologen, 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl) and 2,6-dichlorphenol-indophenol have relative activities of 100%, 19%, 5%, and 1.3%, respectively | Megalodesulfovibrio gigas | acetate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | benzaldehyde + H2O + benzylviologen | 16% of activity with acetaldehyde | Megalodesulfovibrio gigas | benzoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | benzaldehyde + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | benzoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | butyraldehyde + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | butanoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | formaldehyde + H2O + benzylviologen | 16% of activity with acetaldehyde | Megalodesulfovibrio gigas | formate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | furfural + H2O + benzylviologen | 77% of activity with acetaldehyde | Megalodesulfovibrio gigas | furfurate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | glutaraldehyde + H2O + benzylviologen | 20% of activity with acetaldehyde | Megalodesulfovibrio gigas | glutarate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | glycoaldehyde + H2O + benzylviologen | 23% of activity with acetaldehyde | Megalodesulfovibrio gigas | glycolate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | hexanal + H2O + benzylviologen | 6% of activity with acetaldehyde | Megalodesulfovibrio gigas | hexanoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | octanal + H2O + benzylviologen | 93% of activity with acetaldehyde | Megalodesulfovibrio gigas | octanoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | p-anisaldehyde + H2O + benzylviologen | 5% of activity with acetaldehyde | Megalodesulfovibrio gigas | p-anisic acid + reduced benzylviologen | - |
? | |
| 1.2.99.7 | pentanal + H2O + benzylviologen | 9% of activity with acetaldehyde | Megalodesulfovibrio gigas | pentanoate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | phenylacetaldehyde + H2O + benzylviologen | 31% of activity with acetaldehyde | Megalodesulfovibrio gigas | phenylacetate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | propionaldehyde + H2O + benzylviologen | 117% of activity with acetaldehyde | Megalodesulfovibrio gigas | propionate + reduced benzylviologen | - |
? | |
| 1.2.99.7 | salicylaldehyde + H2O + benzylviologen | 14% of activity with acetaldehyde | Megalodesulfovibrio gigas | salicylate + reduced benzylviologen | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.99.6 | dimer | 2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
| 1.2.99.7 | dimer | 2 * 62000, SDS-PAGE | Megalodesulfovibrio gigas |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.99.7 | BV-AIDH | - |
Megalodesulfovibrio gigas |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.6 | 48 | - |
and above | Megalodesulfovibrio gigas |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.7 | 30 | 65 | - |
Megalodesulfovibrio gigas |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.6 | 65 | - |
decrease of activity after 3 min | Megalodesulfovibrio gigas |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.99.6 | 8 | - |
broad, with benzyl viologen and acetaldehyde as substrates | Megalodesulfovibrio gigas |
| 1.2.99.7 | 8 | - |
broad optimum | Megalodesulfovibrio gigas |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.99.6 | molybdopterin | enzyme contains molybdopterin | Megalodesulfovibrio gigas | |
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