EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.25 | p-nitrophenyl-alpha-D-mannopyranoside | activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM | Hordeum vulgare |
EC Number | General Stability | Organism |
---|---|---|
3.2.1.25 | no inactivation by freezing and thawing | Hordeum vulgare |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.25 | 2-amino-2-deoxy-D-mannose | competitive | Hordeum vulgare | |
3.2.1.25 | p-nitrophenyl-alpha-D-mannopyranoside | activates at low concentration, competitively inhibits at higher concentrations, 2-3 mM | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.25 | 0.32 | - |
p-nitrophenyl-beta-D-mannoside | - |
Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.25 | 88000 | - |
x * 88000, SDS-PAGE | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.25 | Hordeum vulgare | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.25 | glycoprotein | 0.01% carbohydrate covalently bound to the partially purified enzyme | Hordeum vulgare |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.25 | - |
Hordeum vulgare |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.25 | 0.506 | - |
- |
Hordeum vulgare |
EC Number | Storage Stability | Organism |
---|---|---|
3.2.1.25 | -10°C, lyophilized enzyme, stable for over a year | Hordeum vulgare |
3.2.1.25 | 4°C, in aqueous solution, stable for several months | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.25 | p-nitrophenyl-beta-D-mannoside + H2O | p-nitrophenyl-beta-D-mannopyranoside | Hordeum vulgare | p-nitrophenol + alpha-D-mannose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.25 | ? | x * 88000, SDS-PAGE | Hordeum vulgare |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.25 | 55 | - |
- |
Hordeum vulgare |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.25 | 5.5 | - |
- |
Hordeum vulgare |