EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.74 | Escherichia coli and Streptomyces lividans | Thermobifida fusca |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.74 | A229V | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | E495D | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | E495N | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G234A | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G234D | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G234N | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G234S | loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose | Thermobifida fusca |
3.2.1.74 | G234S/G284P | loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside | Thermobifida fusca |
3.2.1.74 | G284A | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G284I | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G284P | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G284P/A229V | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G331A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | G496P | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | H326A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | H326S | active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose | Thermobifida fusca |
3.2.1.74 | L230C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | L230C/D512C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | N233C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | N233C/D506C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | W329C | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | W332A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
3.2.1.74 | W332Y | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.74 | D-cellobiose | competitive inhibition | Thermobifida fusca |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.74 | extracellular | - |
Thermobifida fusca | - |
- |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.74 | Thermobifida fusca | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.74 | culture medium | - |
Thermobifida fusca | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.74 | 4-methylumbelliferyl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O | - |
Thermobifida fusca | ? | - |
? | |
3.2.1.74 | 4-methylumbelliferyl beta-cellobioside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + cellobiose | - |
? | |
3.2.1.74 | 4-methylumbelliferyl beta-cellotrioside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + cellotriose | - |
? | |
3.2.1.74 | 4-methylumbelliferyl beta-D-glucopyranoside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + D-glucopyranose | - |
? | |
3.2.1.74 | carboxymethyl cellulose + H2O | - |
Thermobifida fusca | ? | - |
? | |
3.2.1.74 | cellopentaose + H2O | - |
Thermobifida fusca | D-glucose + ? | - |
? | |
3.2.1.74 | cellotetraose + H2O | - |
Thermobifida fusca | D-glucose + cellotriose + cellobiose | - |
? | |
3.2.1.74 | cellotriose + H2O | - |
Thermobifida fusca | D-glucose + cellobiose | - |
? | |
3.2.1.74 | cellulose + H2O | H3PO4 swollen | Thermobifida fusca | D-glucose + ? | - |
? | |
3.2.1.74 | cellulose + H2O | bacterial microcrystalline cellulose | Thermobifida fusca | D-glucose + ? | - |
? |