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Literature summary extracted from

  • Maupin-Furlow, J.A.; Aldrich, H.C.; Ferry, J.G.
    Biochemical characterization of the 20S proteasome from the methanoarchaeon Methanosarcina thermophila (1998), J. Bacteriol., 180, 1480-1487.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.25.1 SDS stimulates chymotrypsin-like activity Methanosarcina thermophila

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.25.1 expression in Escherichia coli Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.25.1 Ca2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 Cl- inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 K+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 Mg2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 Mn2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 SDS inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
3.4.25.1 Zn2+ inhibits peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.25.1 Ca2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
3.4.25.1 Cl- stimulates chymotrypsin-like activity Methanosarcina thermophila
3.4.25.1 K+ stimulates chymotrypsin-like activity Methanosarcina thermophila
3.4.25.1 Mg2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
3.4.25.1 Mn2+ stimulates chymotrypsin-like activity Methanosarcina thermophila
3.4.25.1 Zn2+ stimulates chymotrypsin-like activity Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.25.1 22000
-
 alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila
3.4.25.1 24000
-
 alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila
3.4.25.1 654000
-
 gel filtration Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
3.4.25.1 Methanosarcina thermophila
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.25.1 cleavage of peptide bonds with very broad specificity chymotrypsin-like activity and peptidylglutamyl peptide hydrolase activity Methanosarcina thermophila
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.25.1 0.0013
-
 substrate succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin Methanosarcina thermophila
3.4.25.1 0.0091
-
 substrate carbobenzoxy-Leu-Leu-Glu-beta-naphthylamide Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.25.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Ala-Phe-Lys-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 benzoyl-Phe-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 benzyloxycarbonyl-Leu-Leu-Glu-beta-naphthylamide + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Gly-Gly-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 succinyl-Ile-Ile-Trp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 t-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?
3.4.25.1 Tyr-Val-Ala-Asp-7-amido-4-methylcoumarin + H2O
-
 Methanosarcina thermophila ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.25.1 polymer alpha, beta, 14 * 24000 + 14 * 22000, SDS-PAGE, alpha7,beta7,beta7,alpha7 in the 20S proteasome Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.25.1 7.8
-
 chymotrypsin-like activity Methanosarcina thermophila