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Literature summary extracted from

  • Mbuyi-Kalala, A.; Schnek, A.G.; Leonis, J.
    Separation and characterization of four enzyme forms of beta-galactosidase from Saccharomyces lactis (1988), Eur. J. Biochem., 178, 437-443.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.1
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG630 Kluyveromyces lactis
3.2.1.23 2.5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG550 Kluyveromyces lactis
3.2.1.23 2.6
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG41 Kluyveromyces lactis
3.2.1.23 12.5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG19 Kluyveromyces lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ 0.32 mM, activates isoenzyme betaG550 Kluyveromyces lactis
3.2.1.23 K+ 0.32 mM KCl, activates isoenzyme betaG550 Kluyveromyces lactis
3.2.1.23 Mg2+ 0.32 mM MgCl2, activates isoenzyme betaG550 and betaG19 Kluyveromyces lactis
3.2.1.23 Na+ 0.32 mM NaCl or NaF, activates isoenzyme betaG550 Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 19000
-
 isoenzyme betaG19, gel filtration Kluyveromyces lactis
3.2.1.23 41000
-
 isoenzyme betaG41, gel filtration Kluyveromyces lactis
3.2.1.23 550000
-
 isoenzyme betaG550, gel filtration Kluyveromyces lactis
3.2.1.23 630000
-
 isoenzyme betaG630, gel filtration Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Kluyveromyces lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23 4 isoenzymes: betaG630, betaG550, betaG41 and betaG19 Kluyveromyces lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces lactis o-nitrophenol + beta-D-galactose
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 1
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG41 Kluyveromyces lactis
3.2.1.23 5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG630 Kluyveromyces lactis
3.2.1.23 888
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG550 Kluyveromyces lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
-
 Kluyveromyces lactis