Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moriguchi, M.; Sakai, K.; Tateyama, R.; Furuta, Y.; Wakayama, M.
    Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3 (1994), J. Ferment. Bioeng., 77, 621-625.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.2 Cu2+ CuCl2, inhibition of glutaminase II Micrococcus luteus
3.5.1.2 EDTA glutaminase I and II Micrococcus luteus
3.5.1.2 FeCl3 glutaminase II Micrococcus luteus
3.5.1.2 Na2SO4 glutaminase I and II Micrococcus luteus
3.5.1.2 PCMB inhibition of glutaminase I but not glutaminase II Micrococcus luteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 4.4
-
 L-Gln glutaminase I Micrococcus luteus
3.5.1.2 6.5
-
 L-Gln glutaminase II Micrococcus luteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.2 NaCl 8-16%, maximal activity of glutaminase I, no effect of glutaminase II Micrococcus luteus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.2 43000
-
 2 * 43000, glutaminase I, SDS-PAGE Micrococcus luteus
3.5.1.2 86000
-
 glutaminase I and II, gel filtration Micrococcus luteus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.2 L-Gln + H2O Micrococcus luteus constitutive enzyme L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O Micrococcus luteus K-3 constitutive enzyme L-Glu + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Micrococcus luteus
-
 glutaminase I and II
-
3.5.1.2 Micrococcus luteus K-3
-
 glutaminase I and II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 purification of glutaminase I, partial purification of glutaminase II Micrococcus luteus

Storage Stability

EC Number Storage Stability Organism
3.5.1.2 0-5°C, pH 7.2, 10 mM Tris-HCl, 10% glycerol, 10 mM MgCl2, glutaminase I and II, stable for 1 month Micrococcus luteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 Gln + H2O
-
 Micrococcus luteus Glu + NH3
-
 ?
3.5.1.2 Gln + H2O
-
 Micrococcus luteus K-3 Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O
-
 Micrococcus luteus L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O constitutive enzyme Micrococcus luteus L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O
-
 Micrococcus luteus K-3 L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + H2O constitutive enzyme Micrococcus luteus K-3 L-Glu + NH3
-
 ?
3.5.1.2 L-Gln + hydroxylamine no activity Micrococcus luteus L-gamma-glutamyl hydroxamate + NH3
-
 ?
3.5.1.2 L-Gln + hydroxylamine no activity Micrococcus luteus K-3 L-gamma-glutamyl hydroxamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.2 dimer 2 * 43000, glutaminase I, SDS-PAGE Micrococcus luteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 50
-
 glutaminase I and II Micrococcus luteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.2 additional information
-
 10% NaCl enhances thermal stability of glutaminase I Micrococcus luteus
3.5.1.2 30
-
 pH 7.5, 10 min, stable in absence of NaCl Micrococcus luteus
3.5.1.2 40
-
 pH 7.5, 10 min, 10% NaCl, glutaminase I and II stable up to Micrococcus luteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 8
-
 glutaminase I Micrococcus luteus
3.5.1.2 8.5
-
 glutaminase II Micrococcus luteus