Literature summary extracted from

  • Rossolini, G.M.; Franceschini, N.; Riccio, M.L.; Mercuri, P.S.; Perilli, M.; Galleni, M.; Frere, J.M.; Amicosante,G.
    Characterisation and sequence of the Chryseobacterium (flavobacterium) meningosepticum carbapenemase: a new molecular class B beta-lactamase showing a broad substrate profile (1998), Biochem. J., 332, 145-152.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6
-
Elizabethkingia meningoseptica

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 1,10-phenanthroline
-
Elizabethkingia meningoseptica
3.5.2.6 EDTA
-
Elizabethkingia meningoseptica
3.5.2.6 pyridine-2,6-dicarboxylic acid
-
Elizabethkingia meningoseptica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.18
-
cefotaxime
-
Elizabethkingia meningoseptica
3.5.2.6 0.032
-
penicillin G
-
Elizabethkingia meningoseptica
3.5.2.6 0.029
-
cephaloridine
-
Elizabethkingia meningoseptica
3.5.2.6 0.37
-
Imipenem
-
Elizabethkingia meningoseptica
3.5.2.6 0.024
-
cefoxitin
-
Elizabethkingia meningoseptica
3.5.2.6 0.066
-
nitrocefin
-
Elizabethkingia meningoseptica
3.5.2.6 0.043
-
6-beta-iodopenicillanate
-
Elizabethkingia meningoseptica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+
-
Elizabethkingia meningoseptica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 26000
-
SDS-PAGE Elizabethkingia meningoseptica

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Elizabethkingia meningoseptica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
Elizabethkingia meningoseptica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 110
-
-
Elizabethkingia meningoseptica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 cephaloridine + H2O
-
Elizabethkingia meningoseptica (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
3.5.2.6 cefotaxime + H2O
-
Elizabethkingia meningoseptica (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cefoxitin + H2O
-
Elizabethkingia meningoseptica (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 imipenem + H2O
-
Elizabethkingia meningoseptica (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
3.5.2.6 nitrocefin + H2O
-
Elizabethkingia meningoseptica (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 penicillin G + H2O
-
Elizabethkingia meningoseptica (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 6-beta-iodopenicillanate + H2O
-
Elizabethkingia meningoseptica (2R,4S)-2-[(R)-carboxy(iodo)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylate
-
?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 monomer 1* 26000, SDS-PAGE Elizabethkingia meningoseptica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 6
-
cefoxitin
-
Elizabethkingia meningoseptica
3.5.2.6 39
-
cefotaxime
-
Elizabethkingia meningoseptica
3.5.2.6 17
-
nitrocefin
-
Elizabethkingia meningoseptica
3.5.2.6 14
-
cephaloridine
-
Elizabethkingia meningoseptica
3.5.2.6 350
-
Imipenem
-
Elizabethkingia meningoseptica
3.5.2.6 300
-
6-beta-iodopenicillanate
-
Elizabethkingia meningoseptica
3.5.2.6 280
-
penicillin G
-
Elizabethkingia meningoseptica