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Literature summary extracted from

  • Mochizuki, K.
    Purification and characterization of 5-oxo-L-prolinase from Paecilomyces varioti F-1, an ATP-dependent hydrolase active with L-2-oxothiazolidine-4-carboxylic acid (1999), Arch. Microbiol., 172, 182-185.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.2.9 NH2OH-HCl 1 mM, relative activity 109% Paecilomyces variotii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.9 AgNO3
-
 Paecilomyces variotii
3.5.2.9 CuSO4
-
 Paecilomyces variotii
3.5.2.9 N-bromosuccinimide
-
 Paecilomyces variotii
3.5.2.9 N-ethylmaleimide
-
 Paecilomyces variotii
3.5.2.9 N-ethylmaleimide
-
 Rattus norvegicus
3.5.2.9 p-hydroxymercuribenzoate
-
 Paecilomyces variotii
3.5.2.9 p-hydroxymercuribenzoate
-
 Rattus norvegicus
3.5.2.9 ZnCl2
-
 Paecilomyces variotii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.9 0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Rattus norvegicus
3.5.2.9 0.002
-
 L-2-oxothiazolidine-4-carboxylic acid
-
 Pseudomonas putida
3.5.2.9 0.005
-
 5-oxo-L-proline
-
 Rattus norvegicus
3.5.2.9 0.043
-
 5-oxo-L-proline
-
 Pseudomonas putida
3.5.2.9 0.07
-
 5-oxoproline
-
 Alcaligenes sp.
3.5.2.9 0.091
-
 ATP 5-oxo-L-proline as substrate Paecilomyces variotii
3.5.2.9 0.17
-
 L-2-Oxothiazolidine-4-carboxylate
-
 Paecilomyces variotii
3.5.2.9 0.21
-
 ATP L-2-oxothiazolidine-4-carboxylate as substrate Paecilomyces variotii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.9 FeSO4 1 mM , relative activity 113% Paecilomyces variotii
3.5.2.9 K+
-
 Rattus norvegicus
3.5.2.9 K+
-
 Paecilomyces variotii
3.5.2.9 K+
-
 Pseudomonas putida
3.5.2.9 Mg2+
-
 Rattus norvegicus
3.5.2.9 Mg2+
-
 Paecilomyces variotii
3.5.2.9 Mn2+
-
 Rattus norvegicus
3.5.2.9 Mn2+ 1 mM MnCl2, relative activity 144% Paecilomyces variotii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.9 140000
-
 2 * 140000, SDS-PAGE Rattus norvegicus
3.5.2.9 140000
-
 2 * 140000, SDS-PAGE Paecilomyces variotii
3.5.2.9 260000
-
 gel filtration Paecilomyces variotii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP Rattus norvegicus
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Paecilomyces variotii
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Pseudomonas putida
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Alcaligenes sp.
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Paecilomyces variotii F-1
-
 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP Alcaligenes sp. F-137
-
 L-glutamate + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.9 Alcaligenes sp.
-
-
-
3.5.2.9 Alcaligenes sp. F-137
-
-
-
3.5.2.9 Paecilomyces variotii
-
-
-
3.5.2.9 Paecilomyces variotii F-1
-
-
-
3.5.2.9 Pseudomonas putida
-
-
-
3.5.2.9 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.9
-
 Paecilomyces variotii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.9 0.69
-
-
 Paecilomyces variotii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Rattus norvegicus L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Paecilomyces variotii L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Pseudomonas putida L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Alcaligenes sp. L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Paecilomyces variotii F-1 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 5-oxo-L-proline + H2O + ATP
-
 Alcaligenes sp. F-137 L-glutamate + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Paecilomyces variotii L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Alcaligenes sp. L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Paecilomyces variotii F-1 L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylate + H2O + ATP
-
 Alcaligenes sp. F-137 L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Rattus norvegicus L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 L-2-oxothiazolidine-4-carboxylic acid + H2O
-
 Pseudomonas putida L-cysteine + ADP + phosphate
-
 ?
3.5.2.9 additional information
-
 Pseudomonas putida ?
-
 ?
3.5.2.9 additional information CTP, TTP and UTP are no substrates Paecilomyces variotii ?
-
 ?
3.5.2.9 additional information CTP, TTP and UTP are no substrates Paecilomyces variotii F-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.9 dimer 2 * 140000, SDS-PAGE Rattus norvegicus
3.5.2.9 dimer 2 * 140000, SDS-PAGE Paecilomyces variotii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.9 50
-
-
 Paecilomyces variotii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.2.9 30 55
-
 Paecilomyces variotii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.9 50
-
 64% initial activity retained at 60°C, loses all initial activity at 70°C Paecilomyces variotii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.9 7 8
-
 Paecilomyces variotii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.2.9 6.5 8.5 most stable at pH 7.5, loses activity rapidly below pH 5.5 and above pH 9 Paecilomyces variotii