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Literature summary extracted from

  • Miyamoto, S.; Ogawa, H.; Shiraki, H.; Nakagawa, H.
    Guanine deaminase from rat brain. Purification, characteristics, and contribution to ammoniagenesis in the brain (1982), J. Biochem., 91, 167-176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.4.3 AMP 0.4 mM, residual enzyme activity 112% Rattus norvegicus
3.5.4.3 ATP 0.4 mM, residual enzyme activity 104% Rattus norvegicus
3.5.4.3 uric acid 0.4 mM, residual enzyme activity 105% Rattus norvegicus
3.5.4.3 xanthine 0.4 mM, residual enzyme activity 110% Rattus norvegicus
3.5.4.3 XMP 0.4 mM, residual enzyme activity 113% Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.3 5-aminoimidazole-4-carboxamide
-
 Rattus norvegicus
3.5.4.3 5-aminoimidazole-4-carboxamide ribonucleoside AICAR Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.4.3 0.011
-
 guanine
-
 Sus sp.
3.5.4.3 0.17
-
 guanine
-
 Rattus norvegicus
3.5.4.3 0.67
-
 8-Azaguanine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.4.3 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.3 50000
-
 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 52000
-
 2 * 52000, brain and liver enzymes, SDS-PAGE Rattus norvegicus
3.5.4.3 105000
-
 gel filtration Rattus norvegicus
3.5.4.3 108000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.3 guanine + H2O Rattus norvegicus
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Sus sp.
-
 xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O Rattus norvegicus Wistar
-
 xanthine + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.3 Rattus norvegicus
-
 rat
-
3.5.4.3 Rattus norvegicus
-
 Wistar strain
-
3.5.4.3 Rattus norvegicus Wistar
-
 Wistar strain
-
3.5.4.3 Sus sp.
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.4.3
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.4.3 brain
-
 Rattus norvegicus
-
3.5.4.3 brain
-
 Sus sp.
-
3.5.4.3 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.4.3 13.7
-
 purified brain enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.5.4.3 0°C, 10 mM phosphate buffer, pH 7.0, 0.1 mM dithiothreitol, can be stored for more than 2 weeks without appreciable loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.3 8-azaguanine + H2O
-
 Rattus norvegicus 8-azaxanthine + NH3
-
 ?
3.5.4.3 8-azaguanine + H2O
-
 Rattus norvegicus Wistar 8-azaxanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Rattus norvegicus xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Sus sp. xanthine + NH3
-
 ?
3.5.4.3 guanine + H2O
-
 Rattus norvegicus Wistar xanthine + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.3 dimer 2 * 50000, SDS-PAGE Sus sp.
3.5.4.3 dimer 2 * 52000, brain and liver enzymes, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.3 7.4 9.4
-
 Rattus norvegicus