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Literature summary extracted from
- Characterization of an inducible nitrilase from a thermophilic bacillus (1999), Extremophiles, 3, 283-291.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.5.1 | L-cystine | 16% activation at 1 mM | Aeribacillus pallidus |  |
| 3.5.5.1 | NaHS | 19% activation at 1 mM | Aeribacillus pallidus | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.5.1 | 4,7-phenanthroline | 34% inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | 4-chloromercuribenzoate | complete inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | 4-hydroxymercuribenzoic acid | strong inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | 5,5'-dithiobis(2-nitrobenzoic acid) | 45% inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | 8-hydroxyquinoline | 36% inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | Ag+ | strong inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | dithiothreitol | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | Fe2+ | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | GSH | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | Hg2+ | strong inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | iodoacetamide | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | iodoacetic acid | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | phenylhydrazine | slight inhibition at 1 mM | Aeribacillus pallidus | |
| 3.5.5.1 | Phenylmercuriacetate | complete inhibition at 1 mM | Aeribacillus pallidus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 0.086 | - |
3-chlorobenzonitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 0.3 | - |
2-Cyanopyridine | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 0.92 | - |
Benzonitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 1.3 | - |
4-chlorobenzonitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 2.7 | - |
3-Cyanopyridine | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 22.5 | - |
4-cyanopyridine | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 41 | - |
4-Chlorobutyronitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 124 | - |
Crotononitrile | - |
Aeribacillus pallidus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | Aeribacillus pallidus | - |
nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | Aeribacillus pallidus | - |
benzoic acid + NH3 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | Aeribacillus pallidus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.5.1 | near homogeneity, chromatography techniques | Aeribacillus pallidus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.5.1 | cell culture | best inducer of enzyme formation: benzonitrile | Aeribacillus pallidus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.5.1 | 2-cyanopyridine + 2 H2O | - |
Aeribacillus pallidus | 2-pyridine carboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-fluorobenzonitrile + H2O | - |
Aeribacillus pallidus | 2-fluorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 2-toluonitrile + H2O | - |
Aeribacillus pallidus | NH3 + 2-methylbenzoate | - |
? | |
| 3.5.5.1 | 3-aminobenzonitrile + H2O | - |
Aeribacillus pallidus | 3-aminobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-chloro-4-fluorobenzonitrile + H2O | - |
Aeribacillus pallidus | 3-chloro-4-fluorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-chlorobenzonitrile + H2O | - |
Aeribacillus pallidus | 3-chlorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-cyanopyridine + 2 H2O | - |
Aeribacillus pallidus | nicotinic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-fluorobenzonitrile + H2O | - |
Aeribacillus pallidus | 3-fluorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-hydroxybenzonitrile + H2O | - |
Aeribacillus pallidus | 3-hydroxybenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-nitrobenzonitrile + H2O | - |
Aeribacillus pallidus | 3-nitrobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 3-toluonitrile + H2O | - |
Aeribacillus pallidus | NH3 + 3-methylbenzoate | - |
? | |
| 3.5.5.1 | 4-chlorobenzonitrile + H2O | - |
Aeribacillus pallidus | 4-chlorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-chlorobutyronitrile + H2O | - |
Aeribacillus pallidus | 4-chlorobutyric acid + NH3 | - |
? | |
| 3.5.5.1 | 4-cyanopyridine + 2 H2O | best substrate | Aeribacillus pallidus | 4-pyridinecarboxylic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-fluorobenzonitrile + H2O | - |
Aeribacillus pallidus | 4-fluorobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-hydroxybenzonitrile + H2O | - |
Aeribacillus pallidus | 4-hydroxybenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-nitrobenzonitrile + H2O | - |
Aeribacillus pallidus | 4-nitrobenzoic acid + NH3 | - |
? | |
| 3.5.5.1 | 4-toluonitrile + H2O | - |
Aeribacillus pallidus | NH3 + 4-methylbenzoate | - |
? | |
| 3.5.5.1 | allylcyanide + H2O | - |
Aeribacillus pallidus | NH3 + but-3-enoic acid | - |
? | |
| 3.5.5.1 | benzonitrile + 2 H2O | - |
Aeribacillus pallidus | benzoic acid + NH3 | - |
? | |
| 3.5.5.1 | crotononitrile + H2O | - |
Aeribacillus pallidus | NH3 + (z)-but-2-en-1-ol | - |
? | |
| 3.5.5.1 | n-butyronitrile + H2O | - |
Aeribacillus pallidus | n-butyric acid + NH3 | - |
? | |
| 3.5.5.1 | n-valeronitrile + H2O | - |
Aeribacillus pallidus | n-valeric acid + NH3 | - |
? | |
| 3.5.5.1 | trans-3-pentenenitrile + H2O | - |
Aeribacillus pallidus | trans-3-pentenoic acid + NH3 | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 65 | - |
- |
Aeribacillus pallidus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 70 | - |
rapid inactivation above | Aeribacillus pallidus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.5.1 | 1370 | - |
3-chlorobenzonitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 11100 | - |
4-Chlorobutyronitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 14400 | - |
Benzonitrile | - |
Aeribacillus pallidus | |
| 3.5.5.1 | 43900 | - |
4-cyanopyridine | - |
Aeribacillus pallidus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 7.6 | - |
- |
Aeribacillus pallidus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.5.1 | 6 | 9 | constant activity within | Aeribacillus pallidus |
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