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Literature summary extracted from

  • Patrick, J.; Lee, N.
    L-Arabinose isomerase (1975), Methods Enzymol., 41B, 453-458.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.4 L-arabitol
-
 Escherichia coli
5.3.1.4 ribitol
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.4 60
-
 L-arabinose
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.4 Co2+ does not restore activity after dialysis with EDTA Escherichia coli
5.3.1.4 Mn2+ restores activity after dialysis against EDTA Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.4 60000
-
 6 * 60000, sedimentation in the ultracentrifuge in presence of 8 M urea or pH 2 phosphate buffer Escherichia coli
5.3.1.4 362000
-
 low speed equilibrium sedimentation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.4 Escherichia coli
-
-
-
5.3.1.4 Escherichia coli
-
 temperature-sensitive mutant
-
5.3.1.4 Escherichia coli B/r
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.4
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.4 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
5.3.1.4 4°C, or lyophilized and stored at -20°C, stable for at least 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.4 D-Fucose slightly less than 10% of the activity on L-arabinose Escherichia coli ?
-
 ?
5.3.1.4 D-Fucose slightly less than 10% of the activity on L-arabinose Escherichia coli B/r ?
-
 ?
5.3.1.4 D-Xylose slightly less than 10% of the activity on L-arabinose Escherichia coli ?
-
 ?
5.3.1.4 D-Xylose slightly less than 10% of the activity on L-arabinose Escherichia coli B/r ?
-
 ?
5.3.1.4 L-arabinose
-
 Escherichia coli L-ribulose
-
 ?
5.3.1.4 L-arabinose
-
 Escherichia coli B/r L-ribulose
-
 ?
5.3.1.4 L-Fucose slightly less than 10% of the activity on L-arabinose Escherichia coli ?
-
 ?
5.3.1.4 L-Fucose slightly less than 10% of the activity on L-arabinose Escherichia coli B/r ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.4 hexamer 6 * 60000, sedimentation in the ultracentrifuge in presence of 8 M urea or pH 2 phosphate buffer Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.4 6 8
-
 Escherichia coli